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          • Primary Antibodies ›
          • ATP5I Antibodies

          Invitrogen

          ATP5I Polyclonal Antibody

          View all (11) ATP5I antibodies

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          Cite ATP5I Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          ATP5I Antibody in Immunocytochemistry (ICC/IF)
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          ATP5I Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          ATP5I Antibody (PA5-116695) in ICC/IF

          Immunofluorescence analysis of ATP5I in U-2 OS cells. Samples were incubated with ATP5I Polyclonal antibody (Product # PA5-116695) using a dilution of 1:100. Blue: DAPI for nuclear staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          ATP5I Antibody in Immunocytochemistry (ICC/IF)
          ATP5I Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATP5I Antibody in Western Blot (WB)
          ATP5I Polyclonal Antibody

          Product Details

          PA5-116695

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant fusion protein containing a sequence corresponding to amino acids 1-69 of human ATP5I (NP_0090311)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.49 mg/mL

          Amount

          49 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2901326

          Product Specific Information

          Positive Samples: U-87MG, LO2, 293T, HeLa

          Immunogen sequence: MVPPVQVSPL IKLGRYSALF LGVAYGATRY NYLKPRAEEE RRIAAEEKKK QDELKRIARE LAEDDSILK

          Target Information

          Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. The two complexes are linked by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled, via a rotary mechanism of the central stalk subunits, with proton translocation across the membrane. ATP5I, also known as mitochondrial ATP synthase subunit E or ATP5K, is a 69 amino acid protein member of the ATPase E subunit family. Localized to the inner membrane of the mitochondria, ATP5I is a part of the F0 complex.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: ATP synthase e chain, mitochondrial; ATP synthase F(0) complex subunit e, mitochondrial; ATP synthase membrane subunit e; ATP synthase subunit e, mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E; ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit e; ATP synthase, H+ transporting, mitochondrial Fo complex subunit E; ATPase subunit e; F1F0-ATP synthase, murine e subunit

          View more View less

          Gene Aliases: 2610008D24Rik; ATP5I; ATP5K; ATP5ME; Lfm-1; Lfm1

          View more View less

          UniProt ID: (Human) P56385, (Rat) P29419, (Mouse) Q06185

          View more View less

          Entrez Gene ID: (Human) 521, (Rat) 140608, (Mouse) 11958

          View more View less

          Function(s)
          protein binding hydrogen ion transmembrane transporter activity macromolecular complex binding proton-transporting ATP synthase activity, rotational mechanism ATPase activity ATP synthase
          Process(es)
          ATP biosynthetic process ion transport ATP synthesis coupled proton transport mitochondrial ATP synthesis coupled proton transport hydrogen ion transmembrane transport ATP metabolic process transport proton transport
          It has to be done as per old AB suggested Products section.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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