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Invitrogen
Connexin 43 Polyclonal Antibody
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Product Details
PA5-19115
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Sheep
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (QPFDFPDDNQNSKK) corresponding to the internal amino acids of GJA1 (aa 333-346).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with canine, human and porcine based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: connexin 43; Connexin-43; Cx-43; Cx43; CXA1; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein alpha 1; gap junction protein, alpha 1, 43kDa; GJA1; ODD; ODOD; SDTY3; Syndactyly type III; unnamed protein product
Gene Aliases: AVSD3; CMDR; CX43; Cxn-43; EKVP; EKVP3; GJA1; GJAL; HLHS1; HSS; ODDD; PPKCA
UniProt ID: (Human) P17302, (Rat) P08050
Entrez Gene ID: (Human) 2697, (Rat) 24392
gap junction channel activity
protein binding
beta-catenin binding
ion transmembrane transporter activity
efflux transmembrane transporter activity
tubulin binding
transmembrane transporter activity
glutathione transmembrane transporter activity
alpha-tubulin binding
gap junction hemi-channel activity
gap junction channel activity involved in cardiac conduction electrical coupling
gap junction channel activity involved in cell communication by electrical coupling
signal transducer activity
receptor binding
SH3 domain binding
protein domain specific binding
PDZ domain binding
beta-tubulin binding
connexin binding
scaffold protein binding
gap junction
establishment of mitotic spindle orientation
cardiac conduction system development
cell communication
signal transduction
cell-cell signaling
spermatogenesis
heart development
protein localization
positive regulation of gene expression
cell communication by electrical coupling
glutamate secretion
gap junction assembly
negative regulation of cell growth
negative regulation of gonadotropin secretion
ion transmembrane transport
glutathione transmembrane transport
maintenance of blood-brain barrier
xenobiotic transport
positive regulation of I-kappaB kinase/NF-kappaB signaling
cell-cell junction organization
bone remodeling
transmembrane transport
bone development
atrial cardiac muscle cell action potential
cell communication by electrical coupling involved in cardiac conduction
microtubule-based transport
positive regulation of cold-induced thermogenesis
export across plasma membrane
negative regulation of trophoblast cell migration
regulation of blood circulation
cellular response to beta-amyloid
positive regulation of vascular smooth muscle cell proliferation
positive regulation of morphogenesis of an epithelium
positive regulation of mesodermal cell differentiation
positive regulation of stem cell proliferation
osteoblast differentiation
in utero embryonic development
neuron migration
negative regulation of endothelial cell proliferation
heart looping
epithelial cell maturation
lens development in camera-type eye
chronic inflammatory response
positive regulation of glomerular filtration
endothelium development
atrial ventricular junction remodeling
apoptotic process
positive regulation of cytosolic calcium ion concentration
adult heart development
regulation of heart contraction
negative regulation of cell proliferation
response to pH
response to glucose
vascular transport
negative regulation of gene expression
cell communication by chemical coupling
positive regulation of cell communication by chemical coupling
ATP transport
regulation of bone mineralization
positive regulation of insulin secretion
response to fluid shear stress
embryonic heart tube development
embryonic digit morphogenesis
skeletal muscle tissue regeneration
response to peptide hormone
positive regulation of osteoblast differentiation
positive regulation of protein catabolic process
positive regulation of striated muscle tissue development
positive regulation of vasoconstriction
positive regulation of vasodilation
regulation of bone remodeling
blood vessel morphogenesis
neuron projection morphogenesis
protein oligomerization
regulation of calcium ion transport
negative regulation of cardiac muscle cell proliferation
milk ejection
regulation of ventricular cardiac muscle cell membrane repolarization
regulation of atrial cardiac muscle cell membrane depolarization
regulation of ventricular cardiac muscle cell membrane depolarization
negative regulation of wound healing
cardiac conduction
cellular response to mechanical stimulus
negative regulation of DNA biosynthetic process
regulation of bicellular tight junction assembly
positive regulation of behavioral fear response
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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