BRIP1 Polyclonal Antibody detects FANCJ protein at cytoplasm and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. BRIP1 Polyclonal Antibody (Product # PA5-29889) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

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Product Details

PA5-29889

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 997 and 1221 of Human FANCJ

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

100 µg

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2547363

Product Specific Information

Recommended positive controls: 293T, A431.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

BRIP1 (BRCA1 Interacting Protein C-terminal Helicase 1) is a gene situated on chromosome 17q22, covering 19 exons within its 184-kb coding region to encode a protein containing 1249 amino acids. BRIP1 is integral to the DNA repair processes, specifically through its interaction with the BRCA1 gene, and is involved in the Fanconi anemia complementation group J (FANC J). Mutations in BRIP1 have been identified, playing a role in cancer predisposition with a notable eightfold increased risk for ovarian cancer and associations with cervical cancer risk through its single nucleotide polymorphisms (SNPs). Amplifications and other mutations of BRIP1 are prevalent in a variety of cancers, and its expression involves significant correlations with copy number variations (CNV) and DNA methylation across numerous tumor types. BRIP1's expression is upregulated in certain contexts, which can contribute to cancer progression, as evidenced in the Omani population with breast cancer. These interactions validate its association with DNA damage repair, cell cycle, and metabolism across multiple cancer types.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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BRIP1 Polyclonal Antibody

BRIP1 Antibody (PA5-29889) in IHC (P)