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PAR1 Antibody (HA500159) in WB
Western blot analysis of PAR1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500159, 1:1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat kidney tissue lysate Lane 2: Mouse kidney tissue lysate.
Product Details
HA500159
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within human PAR1 aa 158-176 (Extracellular domain).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.4, with 0.2% BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Target Information
Thrombin is a serine protease that is involved in platelet aggregation and blood coagulation. It is cleaved from its precursor, prothrombin, and converts fibrinogen to fibrin in the final step of the clotting cascade. Thrombin mediates its regulatory effects by activating cell surface G-protein coupled receptors which include PAR-1, PAR-2 and PAR3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C HTR; coagulation factor II (thrombin) receptor; Coagulation factor II receptor; PAR-1; protease activated receptor 1; protease-activated receptor 1; Proteinase-activated receptor 1; Thrombin receptor
Gene Aliases: AI482343; CF2R; F2R; HTR; PAR-1; PAR1; ThrR; TR; TRGPC
UniProt ID: (Human) P25116, (Mouse) P30558
Entrez Gene ID: (Human) 2149, (Mouse) 14062, (Rat) 25439
activation of MAPKK activity
connective tissue replacement involved in inflammatory response wound healing
negative regulation of glomerular filtration
activation of cysteine-type endopeptidase activity involved in apoptotic process
inflammatory response
G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
protein kinase C-activating G-protein coupled receptor signaling pathway
tyrosine phosphorylation of STAT protein
STAT protein import into nucleus
establishment of synaptic specificity at neuromuscular junction
blood coagulation
positive regulation of cell proliferation
negative regulation of cell proliferation
response to wounding
anatomical structure morphogenesis
positive regulation of phosphatidylinositol 3-kinase signaling
platelet activation
regulation of blood coagulation
positive regulation of blood coagulation
positive regulation of cell migration
response to lipopolysaccharide
regulation of interleukin-1 beta production
positive regulation of collagen biosynthetic process
positive regulation of Rho protein signal transduction
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of MAPK cascade
negative regulation of neuron apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of vasoconstriction
positive regulation of smooth muscle contraction
positive regulation of JAK-STAT cascade
homeostasis of number of cells within a tissue
release of sequestered calcium ion into cytosol
positive regulation of release of sequestered calcium ion into cytosol
positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway
positive regulation of calcium ion transport
regulation of sensory perception of pain
platelet dense granule organization
positive regulation of ERK1 and ERK2 cascade
thrombin receptor signaling pathway
negative regulation of renin secretion into blood stream
positive regulation of interleukin-8 secretion
positive regulation of interleukin-6 secretion
signal transduction
hemostasis
obsolete death
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