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Invitrogen
PCNA Polyclonal Antibody
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Product Details
PA5-111000
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Protein corresonding to Human PCNA. Recombinant protein control fragment (Product #RP-95287).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: KCAGNEDIIT LRAEDNADTL ALVFEAPNQE KVSDYEMKLM DLDVEQLGIP EQEYSCVVKM PSGEFARICR DLSHIGDAVV ISCA
Highest antigen sequence indentity to the following orthologs: Mouse - 99%, Rat - 100%.
Target Information
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen
Gene Aliases: ATLD2; PCNA; Pcna/cyclin; PCNAR
UniProt ID: (Human) P12004, (Rat) P04961, (Mouse) P17918
Entrez Gene ID: (Human) 5111, (Rat) 25737, (Mouse) 18538
purine-specific mismatch base pair DNA N-glycosylase activity
chromatin binding
damaged DNA binding
protein binding
enzyme binding
estrogen receptor binding
DNA polymerase processivity factor activity
receptor tyrosine kinase binding
dinucleotide insertion or deletion binding
MutLalpha complex binding
histone acetyltransferase binding
identical protein binding
DNA polymerase binding
transcription factor binding
DNA binding
DNA polymerase processivity factor
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
telomere maintenance via recombination
telomere maintenance
DNA strand elongation involved in DNA replication
leading strand elongation
transcription-coupled nucleotide-excision repair
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA gap filling
mismatch repair
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
heart development
cell proliferation
protein sumoylation
translesion synthesis
epithelial cell differentiation
replication fork processing
positive regulation of deoxyribonuclease activity
nucleotide-excision repair, DNA incision
response to lipid
cellular response to UV
error-prone translesion synthesis
DNA damage response, detection of DNA damage
positive regulation of DNA repair
positive regulation of DNA replication
response to cadmium ion
error-free translesion synthesis
mitotic telomere maintenance via semi-conservative replication
negative regulation of transcription from RNA polymerase II promoter
regulation of DNA replication
base-excision repair, gap-filling
cellular response to xenobiotic stimulus
DNA replication
DNA repair
cellular response to DNA damage stimulus
regulation of catalytic activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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