Western blot analysis of PDK1 using A) 30 µg 293T whole cell extract and B) 30 µg whole cell extract of human PDK1 transfected 293T cells. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PDK1 polyclonal antibody (Product # PA5-28342) at a dilution of 1:10000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 62 and 432 of Human PDK1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28342 targets PDK1 in WB applications and shows reactivity with Human samples.
The PA5-28342 immunogen is recombinant fragment corresponding to a region within amino acids 62 and 432 of Human PDK1.
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.
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