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PERP Antibody (PA5-19980) in ICC/IF
Immunocytochemistry of A431 cells using PERP Polyclonal Antibody (Product # PA5-19980) at 10 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
Product Details
PA5-19980
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to amino acids near the carboxy terminus of human PERP
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Anitgen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11155442
Product Specific Information
In Western blot applications, this antibody detects a band at ~21kDa. A suggested positive control is A431 cell lysate.
PA5-19980 can be used with blocking peptide PEP-0104.
Target Information
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1110017A08Rik; HGNC:17637; KCP-1; keratinocyte associated protein 1; Keratinocyte-associated protein 1; keratinocytes associated protein 1; p53 apoptosis effector related to PMP-22; p53 apoptosis effector related to PMP22; P53-induced protein PIGPC1; RP3-496H19.1; Transmembrane protein THW
Gene Aliases: dJ496H19.1; KCP1; KRTCAP1; PERP; PIGPC1; THW
UniProt ID: (Human) Q96FX8
Entrez Gene ID: (Human) 64065
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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