Proteintech

PIP5K1A Polyclonal Antibody

Product Image Gallery

PIP5K1A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using PIP5K1A antibody 15713-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

PIP5K1A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 15713-1-AP ( PIP5K1A Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PIP5K1A Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 15713-1-AP (PIP5K1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PIP5K1A Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with .5ug PIP5K1A antibody (15713-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IGG with dilution 1:100.

PIP5K1A Antibody in Immunoprecipitation (IP) — IP result of anti-PIP5K1A (IP:15713-1-AP, 4 ug; Detection:15713-1-AP 1:500) with mouse heart tissue lysate 3200 ug.

Product Details

15713-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 4 publications 4 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 1 publication 1 publication
Flow Cytometry (Flow)	0.5 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PIP5K1A Fusion Protein Ag8340 (151-500 aa encoded by BC007833) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.22 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: SLCSEPLIE LCSSGASGSL FYVSSDDEFI IKTVQHKEAE FLQKLLPGYY MNLNQNPRTL LPKFYGLYCV QAGGKNIRIV VMNNLLPRSV KMHIKYDLKG STYKRRASQK EREKPLPTFK DLDFLQDIPD GLFLDADMYN ALCKTLQRDC LVLQSFKIMD YSLLMSIHNI DHAQREPLSS ETQYSVDTRR PAPQKALYST AMESIQGEAR RGGTMETDDH MGGIPARNSK GERLLLYIGI IDILQSYRFV KKLEHSWKAL VHDGDTVSVH RPGFYAERFQ RFMCNTVFKK IPCVHLGRPD VLPQTPPLEE ISEGSPIPDP SFSPLVGETL QMLTTSTTLE KLEVAESEFT H (151-500 aa encoded by BC007833)

Target Information

Overexpression of phosphatidylinositol phosphate 5-kinase alpha (PIP5KIalpha), which synthesizes PIP2, suppresses apoptosis, whereas a kinase-deficient mutant does not. Protection by the wild-type PIP5KIalpha isaccompanied by decreases in the generation of activated caspases and of caspase 3-cleaved PARP. Protection is not mediated through PIP3 or Akt activation. An anti-apoptotic role for PIP(2) is substantiated by the finding that PIP5KIalpha is cleaved by caspase 3 during apoptosis, and cleavage inactivates PIP5KIalpha in vitro. Mutation of the P(4) position (D279A) of the PIP5KIalpha caspase 3 cleavage consensus prevents cleavage in vitro, and during apoptosis in vivo. Significantly, the caspase 3-resistant PIP5KIalpha mutant is more effective in suppressing apoptosis than the wild-type kinase. PIP2 is a direct regulator of apical and effector caspases in the death receptor and mitochondrial pathways, and PIP5KIalpha inactivation contributes to the progression of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The role of PIP5K1α/pAKT and targeted inhibition of growth of subtypes of breast cancer using PIP5K1α inhibitor.

Oncogene

Sarwar M,Syed Khaja AS,Aleskandarany M,Karlsson R,Althobiti M,Ødum N,Mongan NP,Dizeyi N,Johnson H,Green AR,Ellis IO,Rakha EA,Persson JL

Tue Jan 01 00:00:00 EST 2019

The role of PIP5K1α/pAKT and targeted inhibition of growth of subtypes of breast cancer using PIP5K1α inhibitor.

Oncogene

Sarwar M,Syed Khaja AS,Aleskandarany M,Karlsson R,Althobiti M,Ødum N,Mongan NP,Dizeyi N,Johnson H,Green AR,Ellis IO,Rakha EA,Persson JL

Tue Jan 01 00:00:00 EST 2019

The role of PIP5K1α/pAKT and targeted inhibition of growth of subtypes of breast cancer using PIP5K1α inhibitor.

Oncogene

Sarwar M,Syed Khaja AS,Aleskandarany M,Karlsson R,Althobiti M,Ødum N,Mongan NP,Dizeyi N,Johnson H,Green AR,Ellis IO,Rakha EA,Persson JL

Tue Jan 01 00:00:00 EST 2019

Targeted suppression of AR-V7 using PIP5K1α inhibitor overcomes enzalutamide resistance in prostate cancer cells.

Oncotarget

Sarwar M,Semenas J,Miftakhova R,Simoulis A,Robinson B,Gjörloff Wingren A,Mongan NP,Heery DM,Johnsson H,Abrahamsson PA,Dizeyi N,Luo J,Persson JL

Tue Sep 27 00:00:00 EDT 2016

Targeted suppression of AR-V7 using PIP5K1α inhibitor overcomes enzalutamide resistance in prostate cancer cells.

Oncotarget

Sarwar M,Semenas J,Miftakhova R,Simoulis A,Robinson B,Gjörloff Wingren A,Mongan NP,Heery DM,Johnsson H,Abrahamsson PA,Dizeyi N,Luo J,Persson JL

Tue Sep 27 00:00:00 EDT 2016

Spastic paraplegia proteins spastizin and spatacsin mediate autophagic lysosome reformation.

The Journal of clinical investigation

Chang J,Lee S,Blackstone C

Mon Dec 01 00:00:00 EST 2014

Clathrin and phosphatidylinositol-4,5-bisphosphate regulate autophagic lysosome reformation.

Nature cell biology

Rong Y,Liu M,Ma L,Du W,Zhang H,Tian Y,Cao Z,Li Y,Ren H,Zhang C,Li L,Chen S,Xi J,Yu L

Sat Sep 01 00:00:00 EDT 2012

Clathrin and phosphatidylinositol-4,5-bisphosphate regulate autophagic lysosome reformation.

Nature cell biology

Rong Y,Liu M,Ma L,Du W,Zhang H,Tian Y,Cao Z,Li Y,Ren H,Zhang C,Li L,Chen S,Xi J,Yu L

Sat Sep 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase; 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha; 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase; 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha; Phosphatidylinositol 4-phosphate 5-kinase type I alpha; Phosphatidylinositol 4-phosphate 5-kinase type I beta; Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha; phosphatidylinositol-4-phosphate 5-kinase; phosphatidylinositol-4-phosphate 5-kinase type I alpha; phosphatidylinositol-4-phosphate 5-kinase type-1 alpha; phosphatidylinositol-4-phosphate 5-kinase, type 1 beta; phosphatidylinositol-4-phosphate 5-kinase, type I, alpha; PI4P5KIbeta; PI51A; PIP5K1-alpha; PIP5KIalpha; ptdIns(4)P-5-kinase 1 alpha

Gene Aliases: PI4P5K-I[a]; PIP5K1-alpha; PIP5K1A; Pip5k1b; PIP5KIalpha; Pipk5a

UniProt ID: (Human) Q99755, (Mouse) P70182

Entrez Gene ID: (Human) 8394, (Mouse) 18720, (Rat) 365865

Molecular Function: kinase