Proteintech

PIP5K1A Polyclonal Antibody

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PIP5K1A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using PIP5K1A antibody 15713-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

PIP5K1A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 15713-1-AP ( PIP5K1A Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PIP5K1A Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 15713-1-AP (PIP5K1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PIP5K1A Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with .5ug PIP5K1A antibody (15713-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IGG with dilution 1:100.

PIP5K1A Antibody in Immunoprecipitation (IP) — IP result of anti-PIP5K1A (IP:15713-1-AP, 4 ug; Detection:15713-1-AP 1:500) with mouse heart tissue lysate 3200 ug.

Product Details

15713-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 5 publications 5 publications

Flow Cytometry (Flow)

0.5 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PIP5K1A Fusion Protein Ag8340 (151-500 aa encoded by BC007833)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: SLCSEPLIE LCSSGASGSL FYVSSDDEFI IKTVQHKEAE FLQKLLPGYY MNLNQNPRTL LPKFYGLYCV QAGGKNIRIV VMNNLLPRSV KMHIKYDLKG STYKRRASQK EREKPLPTFK DLDFLQDIPD GLFLDADMYN ALCKTLQRDC LVLQSFKIMD YSLLMSIHNI DHAQREPLSS ETQYSVDTRR PAPQKALYST AMESIQGEAR RGGTMETDDH MGGIPARNSK GERLLLYIGI IDILQSYRFV KKLEHSWKAL VHDGDTVSVH RPGFYAERFQ RFMCNTVFKK IPCVHLGRPD VLPQTPPLEE ISEGSPIPDP SFSPLVGETL QMLTTSTTLE KLEVAESEFT H (151-500 aa encoded by BC007833)

Target Information

Overexpression of phosphatidylinositol phosphate 5-kinase alpha (PIP5KIalpha), which synthesizes PIP2, suppresses apoptosis, whereas a kinase-deficient mutant does not. Protection by the wild-type PIP5KIalpha isaccompanied by decreases in the generation of activated caspases and of caspase 3-cleaved PARP. Protection is not mediated through PIP3 or Akt activation. An anti-apoptotic role for PIP(2) is substantiated by the finding that PIP5KIalpha is cleaved by caspase 3 during apoptosis, and cleavage inactivates PIP5KIalpha in vitro. Mutation of the P(4) position (D279A) of the PIP5KIalpha caspase 3 cleavage consensus prevents cleavage in vitro, and during apoptosis in vivo. Significantly, the caspase 3-resistant PIP5KIalpha mutant is more effective in suppressing apoptosis than the wild-type kinase. PIP2 is a direct regulator of apical and effector caspases in the death receptor and mitochondrial pathways, and PIP5KIalpha inactivation contributes to the progression of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Bacterial protein MakA causes suppression of tumour cell proliferation via inhibition of PIP5K1α/Akt signalling.

Cell death & disease

Toh E,Baryalai P,Nadeem A,Aung KM,Chen S,Persson K,Persson JL,Uhlin BE,Wai SN

Tue Dec 06 00:00:00 EST 2022

Bacterial protein MakA causes suppression of tumour cell proliferation via inhibition of PIP5K1α/Akt signalling.

Cell death & disease

Toh E,Baryalai P,Nadeem A,Aung KM,Chen S,Persson K,Persson JL,Uhlin BE,Wai SN

Tue Dec 06 00:00:00 EST 2022

Bacterial protein MakA causes suppression of tumour cell proliferation via inhibition of PIP5K1α/Akt signalling.

Cell death & disease

Toh E,Baryalai P,Nadeem A,Aung KM,Chen S,Persson K,Persson JL,Uhlin BE,Wai SN

Tue Dec 06 00:00:00 EST 2022

Bacterial protein MakA causes suppression of tumour cell proliferation via inhibition of PIP5K1α/Akt signalling.

Cell death & disease

Toh E,Baryalai P,Nadeem A,Aung KM,Chen S,Persson K,Persson JL,Uhlin BE,Wai SN

Tue Dec 06 00:00:00 EST 2022

Proteomic analysis reveals zinc-finger CCHC-type containing protein 3 as a factor inhibiting virus infection by promoting innate signaling.

Virus research

Chen X,Li Z,Wang S,Tong G,Chen K,Zhao Y

Sun Oct 02 00:00:00 EDT 2022

β2-Microglobulin Maintains Glioblastoma Stem Cells and Induces M2-like Polarization of Tumor-Associated Macrophages.

Cancer research

Li D,Zhang Q,Li L,Chen K,Yang J,Dixit D,Gimple RC,Ci S,Lu C,Hu L,Gao J,Shan D,Li Y,Zhang J,Shi Z,Gu D,Yuan W,Wu Q,Yang K,Zhao L,Qiu Z,Lv D,Gao W,Yang H,Lin F,Wang Q,Man J,Li C,Tao W,Agnihotri S,Qian X,Shi Y,You Y,Zhang N,Rich JN,Wang X

Fri Sep 16 00:00:00 EDT 2022

β2-Microglobulin Maintains Glioblastoma Stem Cells and Induces M2-like Polarization of Tumor-Associated Macrophages.

Cancer research

Fri Sep 16 00:00:00 EDT 2022

β2-Microglobulin Maintains Glioblastoma Stem Cells and Induces M2-like Polarization of Tumor-Associated Macrophages.

Cancer research

Fri Sep 16 00:00:00 EDT 2022

PIP5K1α is Required for Promoting Tumor Progression in Castration-Resistant Prostate Cancer.

Frontiers in cell and developmental biology

Wang T,Sarwar M,Whitchurch JB,Collins HM,Green T,Semenas J,Ali A,Roberts CJ,Morris RD,Hubert M,Chen S,El-Schich Z,Wingren AG,Grundström T,Lundmark R,Mongan NP,Gunhaga L,Heery DM,Persson JL

Fri Apr 08 00:00:00 EDT 2022

PIP5K1α is Required for Promoting Tumor Progression in Castration-Resistant Prostate Cancer.

Frontiers in cell and developmental biology

Wang T,Sarwar M,Whitchurch JB,Collins HM,Green T,Semenas J,Ali A,Roberts CJ,Morris RD,Hubert M,Chen S,El-Schich Z,Wingren AG,Grundström T,Lundmark R,Mongan NP,Gunhaga L,Heery DM,Persson JL

Fri Apr 08 00:00:00 EDT 2022

Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKHM1 membrane cycling during autophagosome-lysosome fusion.

The EMBO journal

Baba T,Toth DJ,Sengupta N,Kim YJ,Balla T

Mon Apr 15 00:00:00 EDT 2019

The role of PIP5K1α/pAKT and targeted inhibition of growth of subtypes of breast cancer using PIP5K1α inhibitor.

Oncogene