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Invitrogen
PKA alpha Polyclonal Antibody
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Product Details
PA5-17626
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Bovine,
C. elegans,
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the carboxy terminal sequence of human PKA C-a
Conjugate
Form
Liquid
Concentration
24 µg/mL
Purification
Antigen affinity chromatography, Protein A
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
It is not recommended to aliquot this antibody.
Target Information
Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C(s); C-alpha-2; C-alpha-S; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1; cAMP-dependent protein kinase catalytic subunit C alpha; MGC102831; MGC48865; PKA; PKA C-alpha; pkac; protein kinase A; protein kinase A catalytic subunit; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; sperm cAMP-dependent protein kinase catalytic subunit
Gene Aliases: Cs-PKA; PKACA; PKCA1; PKCD; PPNAD4; PRKACA
UniProt ID: (Human) P17612, (Mouse) P05132
Entrez Gene ID: (Human) 5566, (Rat) 25636, (Mouse) 18747
protein serine/threonine kinase activity
cAMP-dependent protein kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
protein kinase binding
ubiquitin protein ligase binding
protein complex binding
protein kinase A regulatory subunit binding
protein kinase activity
Rab GTPase binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
G2/M transition of mitotic cell cycle
mesoderm formation
neural tube closure
regulation of heart rate
stimulatory C-type lectin receptor signaling pathway
renal water homeostasis
mRNA processing
protein phosphorylation
blood coagulation
positive regulation of cell proliferation
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
activation of protein kinase A activity
calcium-mediated signaling using intracellular calcium source
lipoprotein metabolic process
regulation of protein binding
regulation of cellular respiration
regulation of osteoblast differentiation
protein autophosphorylation
positive regulation of protein export from nucleus
sperm capacitation
negative regulation of meiotic cell cycle
regulation of cytosolic calcium ion concentration
regulation of synaptic transmission, glutamatergic
regulation of cardiac muscle contraction
regulation of ryanodine-sensitive calcium-release channel activity
regulation of proteasomal protein catabolic process
regulation of protein processing
positive regulation of cell cycle arrest
cellular response to glucose stimulus
cellular response to parathyroid hormone stimulus
cellular response to glucagon stimulus
cellular response to epinephrine stimulus
cell communication by electrical coupling involved in cardiac conduction
negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning
regulation of cardiac conduction
regulation of bicellular tight junction assembly
modulation of synaptic transmission
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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