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Proteintech
POLQ Polyclonal Antibody
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Product Details
28590-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
POLQ fusion protein Ag29643
Conjugate
Form
Liquid
Concentration
0.45 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
Product Specific Information
Immunogen sequence: LSHDRRLIQV LNTGADVFRS IAAEWKMIEP ESVGDDLRQQ AKQICYGIIY GMGAKSLGEQ MGIKENDAAC YIDSFKSRYT GINQFMTETV KNCKRDGFVQ TILGRRRYLP GIKDNNPYRK AHAERQAINT IVQGSAADIV KIATVNIQKQ LETFHSTFKS HGHREGMLQS DQTGLSRKRK LQGMFCPIRG GFFILQLHDE LLYEVAEEDV VQVAQIVKNE MESAVKLSVK LKVKVKIGAS WGELKDFDV
Target Information
DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed:25642963, PubMed:25643323). MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed:25642963, PubMed:25643323). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed:25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions. The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ (PubMed:25643323). The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates (PubMed:18503084, PubMed:21050863, PubMed:22135286). Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (PubMed:14576298, PubMed:18503084, PubMed:19188258, PubMed:24648516). Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA polymerase eta; DNA polymerase theta; EC 2.7.7.7; polymerase (DNA directed), theta; polymerase (DNA) theta
Gene Aliases: POLH; POLQ; PRO0327
UniProt ID: (Human) O75417
Entrez Gene ID: (Human) 10721, (Rat) 288079
double-strand break repair via homologous recombination
DNA-dependent DNA replication
DNA repair
base-excision repair
double-strand break repair
cellular response to DNA damage stimulus
somatic hypermutation of immunoglobulin genes
protein homooligomerization
DNA biosynthetic process
double-strand break repair via alternative nonhomologous end joining
negative regulation of double-strand break repair via homologous recombination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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