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Invitrogen

Phospho-A-Raf/B-Raf/C-Raf (Ser446, Ser338, Ser299) Polyclonal Antibody

View all (2) A-Raf/B-Raf/C-Raf antibodies

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Additional Information:

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Product Details

PA5-143756

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-B-Raf (Ser-446) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding serine 446 in human B-Raf. This sequence is conserved in B-Rafs from many species, and has high homology to C-Raf (Ser-338) and A-Raf (Ser-299).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Amount

25 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2942984

Product Specific Information

This antibody was cross-adsorbed to phospho-B-Raf (Ser-579) peptide before affinity purification using phospho-B-Raf (Ser-446) peptide. The purified antibody detects a strong band at 95 kDa* and a weaker band at 105 kDa in western blots of human Jurkat or A431 cells treated with Calyculin A. The antibody also detects other phosphorylated proteins at 150 and 200 kDa in calyculin A treated cells.

Target Information

The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (Ser259)/B-Raf(Ser365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. In B-Raf, multiple phosphorylation sites have been identified, but their specific roles are uncertain. Phosphorylation of Ser-446 may prime B-Raf for activation, and Ser-446 and/or Ser-447 phosphorylation may be critical for B-Raf biological activity during PC12 differentiation. Ser-579 is required for growth factor activation and kinase activity. Thus, multiple sites of phosphorylation within Rafs may be important for regulation of their activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf serine/threonine-protein; Braf; Braf transforming; BRAF1; C-RAF; C-Raf proto-oncogene, serine/threonine kinase; CRaf; Craf1 transforming; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); murine sarcoma 3611 oncogene 1; murine sarcoma viral (v-raf) oncogene homolog B1; Oncogene RAF1; proto-oncogene B-Raf; proto-oncogene c-RAF; raf protein; raf proto-oncogene serine/threonine protein kinase; Raf1; RAFB1; serine/threonine protein kinase; serine/threonine protein kinase/signal transducer; unnamed protein product; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; v-raf-leukemia viral oncogene 1

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Gene Aliases: 6430402F14Rik; 9930012E13Rik; B-raf; B-RAF1; BRAF-1; Braf-2; BRAF1; Braf2; c-Raf; C230098H17; CMD1NN; CRAF; Craf1; D6Ertd631e; D830050J10Rik; NS5; NS7; Raf-1; RAFB1; v-Raf

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UniProt ID: (Human) P15056, (Mouse) P28028, (Human) P04049, (Mouse) Q99N57, (Rat) P11345

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Entrez Gene ID: (Human) 673, (Rat) 114486, (Mouse) 109880, (Human) 5894, (Mouse) 110157, (Rat) 24703

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Function(s)
protein kinase activity protein serine/threonine kinase activity MAP kinase kinase activity MAP kinase kinase kinase activity calcium ion binding protein binding ATP binding small GTPase binding identical protein binding scaffold protein binding protein serine kinase activity nucleotide binding mitogen-activated protein kinase kinase binding macromolecular complex binding adenylate cyclase activator activity enzyme binding adenylate cyclase binding
Process(es)
MAPK cascade myeloid progenitor cell differentiation protein phosphorylation signal transduction epidermal growth factor receptor signaling pathway visual learning positive regulation of gene expression negative regulation of fibroblast migration positive regulation of glucose transport cell differentiation thyroid gland development positive regulation of peptidyl-serine phosphorylation somatic stem cell population maintenance regulation of cell proliferation negative regulation of apoptotic process CD4-positive, alpha-beta T cell differentiation positive T cell selection CD4-positive or CD8-positive, alpha-beta T cell lineage commitment response to peptide hormone negative regulation of neuron apoptotic process regulation of T cell differentiation alpha-beta T cell differentiation thymus development regulation of axon regeneration positive regulation of axon regeneration positive regulation of axonogenesis T cell receptor signaling pathway positive regulation of stress fiber assembly response to cAMP long-term synaptic potentiation head morphogenesis face development positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion cellular response to xenobiotic stimulus establishment of protein localization to membrane postsynaptic modulation of chemical synaptic transmission positive regulation of substrate adhesion-dependent cell spreading cellular response to nerve growth factor stimulus negative regulation of synaptic vesicle exocytosis negative regulation of endothelial cell apoptotic process organ morphogenesis response to hypoxia cellular glucose homeostasis positive regulation of protein phosphorylation neuromuscular junction development negative regulation of cell proliferation insulin receptor signaling pathway Schwann cell development negative regulation of protein complex assembly intracellular signal transduction insulin secretion involved in cellular response to glucose stimulus response to muscle stretch ERBB signaling pathway ERBB2-ERBB3 signaling pathway myelination positive regulation of MAPK cascade intermediate filament cytoskeleton organization positive regulation of transcription from RNA polymerase II promoter insulin-like growth factor receptor signaling pathway neurotrophin TRK receptor signaling pathway death-inducing signaling complex assembly negative regulation of extrinsic apoptotic signaling pathway via death domain receptors heart development apoptotic process regulation of Rho protein signal transduction wound healing regulation of apoptotic process regulation of cell differentiation interferon-gamma-mediated signaling pathway
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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