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Invitrogen

Phospho-A-Raf/B-Raf/C-Raf (Ser446, Ser338, Ser299) Polyclonal Antibody

View all (2) A-Raf/B-Raf/C-Raf antibodies

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Product Details

PA5-143756

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-B-Raf (Ser-446) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding serine 446 in human B-Raf. This sequence is conserved in B-Rafs from many species, and has high homology to C-Raf (Ser-338) and A-Raf (Ser-299).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Amount

25 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2942984

Product Specific Information

This antibody was cross-adsorbed to phospho-B-Raf (Ser-579) peptide before affinity purification using phospho-B-Raf (Ser-446) peptide. The purified antibody detects a strong band at 95 kDa* and a weaker band at 105 kDa in western blots of human Jurkat or A431 cells treated with Calyculin A. The antibody also detects other phosphorylated proteins at 150 and 200 kDa in calyculin A treated cells.

Target Information

The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (Ser259)/B-Raf(Ser365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. In B-Raf, multiple phosphorylation sites have been identified, but their specific roles are uncertain. Phosphorylation of Ser-446 may prime B-Raf for activation, and Ser-446 and/or Ser-447 phosphorylation may be critical for B-Raf biological activity during PC12 differentiation. Ser-579 is required for growth factor activation and kinase activity. Thus, multiple sites of phosphorylation within Rafs may be important for regulation of their activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf serine/threonine-protein; BRAF1; C-RAF; C-Raf proto-oncogene, serine/threonine kinase; CRaf; Craf1 transforming; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); murine sarcoma 3611 oncogene 1; murine sarcoma viral (v-raf) oncogene homolog B1; Oncogene RAF1; p94; Proto-oncogene B-Raf; Proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; Raf1; RAFB1; sarcoma 3611 oncogene; serine/threonine protein kinase; Serine/threonine-protein kinase B-raf; unnamed protein product; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1; v-raf-1 leukemia viral oncogene 1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1

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Gene Aliases: 6430402F14Rik; 9930012E13Rik; AA120551; AA387315; AA473386; AA990557; B-raf; B-RAF1; BB129353; BRAF; BRAF-1; Braf-2; BRAF1; Braf2; c-Raf; C230098H17; C87398; CMD1NN; CRAF; Craf1; D6Ertd631e; D830050J10Rik; NS5; NS7; RAF; Raf-1; RAF1; RAFB1; v-Raf

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UniProt ID: (Human) P15056, (Mouse) P28028, (Human) P04049, (Mouse) Q99N57, (Rat) P11345

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Entrez Gene ID: (Human) 673, (Rat) 114486, (Mouse) 109880, (Human) 5894, (Mouse) 110157, (Rat) 24703

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Function(s)
nucleotide binding catalytic activity protein kinase activity protein serine/threonine kinase activity MAP kinase kinase activity MAP kinase kinase kinase activity calcium ion binding protein binding ATP binding zinc ion binding kinase activity transferase activity small GTPase binding identical protein binding metal ion binding scaffold protein binding protein serine kinase activity mitogen-activated protein kinase kinase binding protein heterodimerization activity receptor signaling protein activity transferase activity, transferring phosphorus-containing groups adenylate cyclase activator activity enzyme binding Ras GTPase binding non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade myeloid progenitor cell differentiation protein phosphorylation signal transduction epidermal growth factor receptor signaling pathway visual learning organ morphogenesis positive regulation of gene expression negative regulation of fibroblast migration positive regulation of glucose transport synaptic vesicle exocytosis cell differentiation thyroid gland development T cell differentiation in thymus positive regulation of peptidyl-serine phosphorylation substrate adhesion-dependent cell spreading somatic stem cell population maintenance regulation of cell proliferation negative regulation of apoptotic process stress fiber assembly CD4-positive, alpha-beta T cell differentiation positive T cell selection CD4-positive or CD8-positive, alpha-beta T cell lineage commitment negative regulation of neuron apoptotic process regulation of T cell differentiation alpha-beta T cell differentiation thymus development regulation of axon regeneration positive regulation of axon regeneration positive regulation of axonogenesis T cell receptor signaling pathway positive regulation of stress fiber assembly long-term synaptic potentiation head morphogenesis face development ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion cellular response to xenobiotic stimulus endothelial cell apoptotic process establishment of protein localization to membrane postsynaptic modulation of chemical synaptic transmission positive regulation of substrate adhesion-dependent cell spreading negative regulation of synaptic vesicle exocytosis negative regulation of endothelial cell apoptotic process activation of MAPKK activity cellular response to drug response to peptide hormone protein heterooligomerization response to cAMP glucose transport phosphorylation intracellular signal transduction cellular glucose homeostasis apoptotic process negative regulation of cell proliferation insulin receptor signaling pathway extrinsic apoptotic signaling pathway via death domain receptors Schwann cell development negative regulation of protein complex assembly regulation of Rho protein signal transduction insulin secretion involved in cellular response to glucose stimulus response to muscle stretch ERBB2-ERBB3 signaling pathway wound healing myelination regulation of apoptotic process positive regulation of MAPK cascade type B pancreatic cell proliferation intermediate filament cytoskeleton organization regulation of cell differentiation positive regulation of transcription from RNA polymerase II promoter insulin-like growth factor receptor signaling pathway neurotrophin TRK receptor signaling pathway interferon-gamma-mediated signaling pathway death-inducing signaling complex assembly negative regulation of extrinsic apoptotic signaling pathway via death domain receptors response to hypoxia positive regulation of protein phosphorylation heart development
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