NeoBiotechnologies

RAD51 Monoclonal Antibody (RAD51, 2702)

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RAD51 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human colon carcinoma using RAD51 Monoclonal Antibody (Product # 5888-MSM2-P1).

RAD51 Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of K562 cells using RAD51 Monoclonal Antibody (Product # 5888-MSM2-P1) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

RAD51 Antibody in Peptide array (ARRAY) — Analysis of Protein Array containing more than 19,000 full-length human proteins RAD51 Monoclonal Antibody (Product # 5888-MSM2-P1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

RAD51 Antibody — SDS-PAGE Analyis Purified RAD51 Monoclonal Antibody (Product # 5888-MSM2-P1). Confirmation of Purity and Integrity of Antibody.

Product Details

5888-MSM2-P1

Applications	Tested Dilution	Publications
Immunohistochemistry (Paraffin) (IHC (P))	1-2 µg/mL	-
Flow Cytometry (Flow)	1-2 µg/mL	-
Peptide Array (Array)	Assay-dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	RAD51, 2702
Immunogen	Recombinant fragment of human RAD51 protein (around aa 1-134) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	200 µg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	4° C

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; HsRAD51; RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A

Gene Aliases: BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA

UniProt ID: (Human) Q06609

Entrez Gene ID: (Human) 5888

Molecular Function: DNA metabolism protein