Applications | Tested Dilution | Publications |
---|---|---|
Immunohistochemistry (Paraffin) (IHC (P)) |
1-2 µg/mL | - |
Flow Cytometry (Flow) |
1-2 µg/mL | - |
Peptide Array (Array) |
Assay-dependent | - |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Host/Isotype |
Mouse / IgG1, kappa |
Class |
Monoclonal |
Type |
Antibody |
Clone |
RAD51, 2702 |
Immunogen |
Recombinant fragment of human RAD51 protein (around aa 1-134) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
200 µg/mL |
Purification |
Protein G |
Storage buffer |
PBS, pH 7.4, with 0.05% BSA |
Contains |
0.05% sodium azide |
Storage conditions |
4° C |
Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; HsRAD51; RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A
Gene Aliases: BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA
UniProt ID: (Human) Q06609
Entrez Gene ID: (Human) 5888
Molecular Function:
DNA metabolism protein
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