Search
Agrisera
RAF2/Rubisco accumulation factor 2 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
RAF2/Rubisco accumulation factor 2 Antibody (AS13-2729) in WB
1-15 µg of chlorophyll from isolated chloroplasts from Arabidopsis thaliana, extracted with a buffer containing (25 m M Tricine-Na OH, p H 7.8,330 m M sorbitol, 1 m M EDTA, 10 m M KCl, 0.15% [w/v] bovine serum albumin, 4 m M sodium ascorbate, and 7 m M L-Cys) were separated on 12% SDS-PAGE and blotted 1 h to PVDF using semi-dry transfer. Blots were blocked with 10% milk for 1 h at room temperature (RT) with... View More
Product Details
AS13-2729
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant, RAF2 protein chosen from Arabidopsis thaliana protein sequence.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
RAF2 is a plant chloroplast protein that functions as a Rubisco assembly factor, promoting proper folding and integration of Rubisco large and small subunits to form an active carboxylase for CO2 fixation. It cooperates with chaperones and assembly partners such as RAF1, RbcX, and chaperonins to stabilize intermediates and ensure efficient biogenesis of Rubisco during chloroplast development and under changing environmental conditions. By supporting Rubisco assembly, RAF2 impacts photosynthetic capacity, redox balance, and growth, and its expression aligns with high photosynthetic demand and light regulated chloroplast maturation. Perturbation of RAF2 reduces Rubisco accumulation and activity, lowering carbon assimilation and biomass, while coordinated regulation of RAF2 helps plants maintain photosynthetic efficiency and recover from stress that challenges protein assembly in the thylakoid and stroma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Atg5 g51110; SDIRIP1
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support