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REF1/APEX1 Polyclonal Antibody
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Product Details
BS-1406R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human APEX1, amino acids 201-318.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions. APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases. APE is differentially expressed during development and in different tissues. This protein has diverse subcellular localization patterns which support the possibility of its interaction with numerous, other cellular proteins in addition to DNA repair within the nucleus. Regulation of APE by phosphorylation is mediated, at least in part, by casein kinase II. Increases in APE message and protein levels are observed upon the reintroduction of oxygen to hypoxic cells, and in some malignant tissue relative to normal tissue. Decreases in APE expression have been associated with the induction of cellular apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AP endonuclease 1; AP endonuclease class I; AP lyase; APEN; APEX nuclease; APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease 1; Apurinic-apyrimidinic endonuclease 1; apurinic/a; Apurinic/apy; apurinic/apyrimidinic (abasic) endonuclease; apurinic/apyrimidinic endonuclease 1; deoxyribonuclease (apurinic or apyrimidinic); DNA repair nuclease/redox regulator APEX1; DNA-(apurinic or apyrimidinic site) endonuclease; DNA-(apurinic or apyrimidinic site) lyase; HAP1; protein REF-1; redox factor; redox factor 1; Redox factor-1; REF-1; unnamed protein product
Gene Aliases: APE; APE1; APEN; APEX; APEX1; APX; HAP1; REF-1; REF1
UniProt ID: (Human) P27695, (Mouse) P28352, (Rat) P43138
Entrez Gene ID: (Human) 328, (Mouse) 11792, (Rat) 79116
DNA binding
damaged DNA binding
double-stranded telomeric DNA binding
transcription coactivator activity
transcription corepressor activity
RNA binding
catalytic activity
DNA-(apurinic or apyrimidinic site) lyase activity
nuclease activity
endonuclease activity
endodeoxyribonuclease activity
RNA-DNA hybrid ribonuclease activity
exonuclease activity
phosphodiesterase I activity
uracil DNA N-glycosylase activity
protein binding
phosphoric diester hydrolase activity
3'-5'-exodeoxyribonuclease activity
double-stranded DNA exodeoxyribonuclease activity
double-stranded DNA 3'-5' exodeoxyribonuclease activity
3'-5' exonuclease activity
oxidoreductase activity
hydrolase activity
chromatin DNA binding
deoxyribonuclease (pyrimidine dimer) activity
metal ion binding
class II DNA-(apurinic or apyrimidinic site) endonuclease activity
phosphodiesterase activity, acting on 3'-phosphoglycolate-terminated DNA strands
DNA-(abasic site) binding
endoribonuclease activity
lyase activity
site-specific endodeoxyribonuclease activity, specific for altered base
protein complex binding
phosphoric ester hydrolase activity
T/G mismatch-specific endonuclease activity
poly(A) RNA binding
retroviral 3' processing activity
NF-kappaB binding
DNA repair
base-excision repair
DNA recombination
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
aging
negative regulation of smooth muscle cell migration
regulation of mRNA stability
cell redox homeostasis
positive regulation of DNA repair
oxidation-reduction process
cellular response to hydrogen peroxide
DNA demethylation
nucleic acid phosphodiester bond hydrolysis
RNA phosphodiester bond hydrolysis, endonucleolytic
positive regulation of G1/S transition of mitotic cell cycle
regulation of nucleic acid-templated transcription
DNA catabolic process, endonucleolytic
DNA catabolic process, exonucleolytic
response to organonitrogen compound
response to drug
cellular response to cAMP
cellular response to peptide hormone stimulus
cellular response to organonitrogen compound
telomere maintenance
base-excision repair, gap-filling
DNA catabolic process
regulation of apoptotic process
hypomethylation of CpG island
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
telomere maintenance via base-excision repair
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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