This antibody can be used to detect native RFP, RFP variants, and RFP fusion proteins in Western blot, immunoprecipitation, ELISA, and immunocytochemistry. This antibody has been shown to detect denatured and native TurboRFP, TurboFP602, TurboFP635, TagRFP, TagFP635, and mKate2.
Storage and reconstitution: to prepare a 1 mg/mL stock solution, add 100 µl sterile water or 50% glycerol to the vial. If desired, aliquot the antibody upon reconstitution to produce single-use samples. Store the reconstituted product with sterile water at 4°C, avoiding freeze/thaw cycles. When stored as directed, the solution is stable for 3 months. If reconstituted with 50% glycerol, store at -20°C, avoiding freeze/thaw cycles. When stored as directed, this solution is stable for 6 months.
Red Fluorescent Protein (RPF) is isolated from sea anemone (Discosoma sp), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions. Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Red fluorescent protein; RFP tag