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HeLa cells were incubated with Organelle Lights™ Mito-RFP (O36229) and Organelle Lights™ Golgi-GFP (O10098). Following fixation and permeabilization, cells were stained with Alexa Fluor® 350 phalloidin (A22281) and anti-RFP rabbit polyclonal antibody (R10367), the latter subsequently visualized with Alexa Fluor® 647 Goat Anti-Rabbit IgG antibody (A21245, red fluorescence) and imaged. No cross-reactivity is observed of the anti-RFP antibody with GFP (green-fluorescence, perinuclear region).
|Tested species reactivity||Tag|
|Published species reactivity||Fruit fly, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full-length recombinant denatured and non-denatured TagRFP was used as the immunogen|
|Storage buffer||PBS, pH 7.4, with 0.25mg/ml gelatin, 1% trehalose|
|Storage Conditions||-20° C, store in dark|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:3000-1:7000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody can be used to detect native RFP, RFP variants, and RFP fusion proteins in Western blot, immunoprecipitation, ELISA, and immunocytochemistry. This antibody has been shown to detect denatured and native TurboRFP, TurboFP602, TurboFP635, TagRFP, TagFP635, and mKate2.
Storage and reconstitution: to prepare a 1 mg/mL stock solution, add 100 ?L sterile water or 50% glycerol to the vial. If desired, aliquot the antibody upon reconstitution to produce single-use samples. Store the reconstituted product with sterile water at 4°C, avoiding freeze/thaw cycles. When stored as directed, the solution is stable for 3 months. If reconstituted with 50% glycerol, store at -20°C, avoiding freeze/thaw cycles. When stored as directed, this solution is stable for 6 months.
Red Fluorescent Protein (RPF) is isolated from sea anemone (Discosoma sp), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions. Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Comprehensive models of human primary and metastatic colorectal tumors in immunodeficient and immunocompetent mice by chemokine targeting.
R10367 was used in immunohistochemistry - paraffin section to describe the use of CCR9 expression to create primary gastrointestinal tumors in immunodeficient mice by tail-vein injection.
|Chen HJ,Sun J,Huang Z,Hou H,Arcilla M,Rakhilin N,Joe DJ,Choi J,Gadamsetty P,Milsom J,Nandakumar G,Longman R,Zhou XK,Edwards R,Chen J,Chen KY,Bu P,Wang L,Xu Y,Munroe R,Abratte C,Miller AD,Gümüş ZH,Shuler M,Nishimura N,Edelmann W,Shen X,Lipkin SM||Nature biotechnology (33:656)||2015|
ATG12-ATG3 interacts with Alix to promote basal autophagic flux and late endosome function.
R10367 was used in western blot to uncover a role for ATG12-ATG3 in late endosome function that is distinct from autophagy.
|Murrow L,Malhotra R,Debnath J||Nature cell biology (17:300)||2015|
|Not Applicable||Not Cited||
A new role for annexin A11 in the early secretory pathway via stabilizing Sec31A protein at the endoplasmic reticulum exit sites (ERES).
R10367 was used in immunocytochemistry to study the role of calcium binding proteins in ER to Golgi trafficking
|Shibata H,Kanadome T,Sugiura H,Yokoyama T,Yamamuro M,Moss SE,Maki M||The Journal of biological chemistry (290:4981)||2015|
Digital in vivo 3D atlas of the antennal lobe of Drosophila melanogaster.
R10367 was used in immunohistochemistry to examine the antennal lobe of Drosophila melanogaster
|Grabe V,Strutz A,Baschwitz A,Hansson BS,Sachse S||The Journal of comparative neurology (523:530)||2015|
|Mouse||Not Cited||Parathyroid hormone-related protein activates Wnt signaling to specify the embryonic mammary mesenchyme.||Hiremath M,Dann P,Fischer J,Butterworth D,Boras-Granic K,Hens J,Van Houten J,Shi W,Wysolmerski J||Development (Cambridge, England) (139:4239)||2012|
Exuberant growth and synapse formation of olfactory sensory neuron axonal arborizations.
R10367 was used in immunohistochemistry to test if olfactory sensory projections are established in an exuberant fashion.
|Marcucci F,Maier-Balough E,Zou DJ,Firestein S||The Journal of comparative neurology (519:3713)||2011|