Invitrogen

ROC1 Polyclonal Antibody

Advanced Verification

IP-mass spectrometry View Details

4 Published Figures

View all (14) ROC1 antibodies

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Questions & Answers

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Questions & Answers

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ROC1 Antibody in IP-MS — IP-MS enrichment of ROC1 (LFQ intensity): ROC1 was enriched 2180-fold from BT549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and ROC1 antibody (Product # 34-2500). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

ROC1 Antibody — Published figure using ROC1 polyclonal antibody (Product # 34-2500)

ROC1 Antibody — Published figure using ROC1 polyclonal antibody (Product # 34-2500)

ROC1 Antibody — Published figure using ROC1 polyclonal antibody (Product # 34-2500)

ROC1 Antibody — Published figure using ROC1 polyclonal antibody (Product # 34-2500)

Product Details

Applications

Tested Dilution

Publications

Immunoprecipitation (IP)

Assay Dependent

-

Western Blot (WB)

Assay Dependent

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Virus

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

specific to Human ROC1C

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533162

Target Information

This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase RBX1; E3 ubiquitin-protein ligase RBX1, N-terminally processed; E3 ubiquitin-protein transferase RBX1; E3 ubiquitin-protein transferase RBX1, N-terminally processed; Protein ZYP; Rbx1; Regulator of cullins 1; RING box protein 1; RING finger protein 75; ring-box 1, E3 ubiquitin protein ligase; RING-box protein 1; ROC1; ZYP protein

Gene Aliases: 1500002P15Rik; AA517855; BA554C12.1; RBX1; RNF75; ROC1

UniProt ID: (Human) P62877, (Mouse) P62878

Entrez Gene ID: (Human) 9978, (Mouse) 56438

Molecular Function: ligase ubiquitin-protein ligase

MAPK cascade protein polyubiquitination nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion protein monoubiquitination viral process Wnt signaling pathway protein ubiquitination SCF-dependent proteasomal ubiquitin-dependent protein catabolic process nucleotide-excision repair, DNA incision DNA damage response, detection of DNA damage protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process protein neddylation regulation of transcription from RNA polymerase II promoter in response to hypoxia global genome nucleotide-excision repair negative regulation of canonical Wnt signaling pathway DNA repair ubiquitin-dependent protein catabolic process cellular response to DNA damage stimulus protein catabolic process

Process(es)

ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity NEDD8 transferase activity ubiquitin protein ligase binding protein complex binding ubiquitin-ubiquitin ligase activity ubiquitin protein ligase activity cullin family protein binding eukaryotic initiation factor 4E binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity