Hydrolysis of recombinant aggrecan interglobular domain with isolated ADAMTS4 Recombinant aggrecan-IGD1 (Product # RP-77527) at a concentration of 10 µM in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2 was incubated without and with 0.1 µM truncated ADAMTS4 (Product # RP-77540) for various time intervals. Aliquots with 1.5 µg aggrecan-IGD1 were then analyzed by SDS-PAGE. Lane 1: Aggrecan-IGD1 incubated without ADAMTS4, Lanes 2-6: Digestion mixtures of aggrecan-IGD1 with ADAMTS4 after 0, 0.5, 1, 2 and 5 hours incubation.

Product Image Gallery

Product Details

RP-77527

Product Specifications

Expression System

E. coli

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Storage conditions

-80°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

RP-77527 is a purified recombinant Aggrecan Interglobular Domain protein. The recombinant protein contains cleavage sites for aggrecanases (E373-A374 in aggrecan) and matrix metalloproteinases (N341-F342 in aggrecan). RP-77527 may be used as a control with corresponding anti-Aggrecan Interglobular Domain antibodies.

Target Information

Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (approximately 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Human Aggrecan (Interglobular Domain) Recombinant Protein

Human Aggrecan (Interglobular Domain) Protein (RP-77527)