|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 22 amino acid peptide near the center of human SHISA9|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||2.5 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
A suggested positive control is rat brain tissue lysate.
PA5-21058 can be used with blocking peptide PEP-1172.
Shisa proteins are a recently-identified family of modulators of both FGF and Wnt signaling that block both maturation and transport to the cell surface of their respective receptors. SHISA9, also known as CKAMP44a, is a brain-specific type I transmembrane protein that associates with AMPA receptors in synaptic spines and promotes AMPA receptor desensitization at excitatory synapses. It is thought to modulate short-term plasticity at specific excitatory synapses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
C-terminal interactors of the AMPA receptor auxiliary subunit Shisa9.
PA5-21058 was used in immunoprecipitation to identify proteins that interact with the C-terminal domain of the Shisa9 auxilliary subunit of the AMPA receptor
|Karataeva AR,Klaassen RV,Ströder J,Ruiperez-Alonso M,Hjorth JJ,van Nierop P,Spijker S,Mansvelder HD,Smit AB||PloS one (9:null)||2014|