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|Tested species reactivity||Human|
|Host / Isotype||Sheep / IgG|
|Immunogen||A synthetic peptide from human Synaptotagmin-1 (Synaptotagmin I) conjugated to an immunogenic carrier protein was used as the immunogen|
|Purification||Ammonium sulfate precipitation|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||10 - 50 µg/ml|
|Western Blot (WB)||10 - 50 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Glycerol (1:1) may be added for additional stability.
Reconstitute in 500 ul of sterile water.
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
SVP65; synaptotagmin I; SytI
P65; SVP65; SYT; SYT1