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Bethyl Laboratories
TOP1 Polyclonal Antibody
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FIGURE: 1 / 1
TOP1 Antibody (A302-589A) in WB
Detection of human TOP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TOP1 antibody (Product # A302-589A) used for WB at 0.04 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 3 µg/mg lysate. TOP1 was less efficiently immunoprecipitated by rabbit anti-TOP1 antibody A302-590A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B)... View More
Product Details
A302-589A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 100 and 150 of Human DNA topoisomerase I.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat
Target Information
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA topoisomerase 1; DNA topoisomerase I; DNA topoisomerase I (EC 5.99.1.2); RP3-511B24.2; Scl-70 antigen; topoisomerase (DNA) I; Topoisomerase 1; topoisomerase I; type I DNA topoisomerase; unnamed protein product
Gene Aliases: TOP1; TOPI
UniProt ID: (Human) P11387
Entrez Gene ID: (Human) 7150
RNA polymerase II core promoter proximal region sequence-specific DNA binding
DNA binding
chromatin binding
double-stranded DNA binding
single-stranded DNA binding
RNA binding
DNA topoisomerase activity
DNA topoisomerase type I activity
protein serine/threonine kinase activity
protein binding
ATP binding
DNA binding, bending
isomerase activity
protein domain specific binding
chromatin DNA binding
macromolecular complex binding
supercoiled DNA binding
DNA replication
DNA topological change
chromatin remodeling
chromosome segregation
circadian rhythm
response to temperature stimulus
rRNA transcription
response to xenobiotic stimulus
response to gamma radiation
programmed cell death
peptidyl-serine phosphorylation
organ regeneration
circadian regulation of gene expression
embryonic cleavage
rhythmic process
response to cAMP
cellular response to luteinizing hormone stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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