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Invitrogen
TRIB1 Polyclonal Antibody
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FIGURE: 1 / 3
TRIB1 Antibody (PA5-14801) in WB
Western blot analysis of TRIB1 in human skeletal muscle lysate. Samples were incubated with TRIB1 polyclonal antibody (Product # PA5-14801) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 41 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Product Details
PA5-14801
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 341-370 amino acids from the C-terminal region of human TRIB1
Conjugate
Form
Liquid
Purification
Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Target Information
TRIB1 also called gprotein coupled receptor induced, skip1, gig2, c8fwATP, functions in binding protein binding, protein kinase inhibitor activity, and protein serine/threonine kinase activities. Overexpression of TRIB1 in HeLa cells repressed the basal activity of the IL8 promoter by inhibiting AP1 activity. Overexpression of TRIB1 inhibited oncogenic Ras -driven AP1 activation and MEKK1-mediated AP1 activation. ERK activation was enhanced by TRIB1. Coimmunoprecipitation and yeast 2-hybrid assays showed that MEK1 interacted with both TRIB1 and TRIB3, and MKK4 interacted specifically with TRIB1. Cotransfection of MKK4 enhanced the level of TRIB1, indicating that the TRIB-MAPKK interaction stabilized TRIB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G-protein-coupled receptor induced protein; G-protein-coupled receptor-induced gene 2 protein; G-protein-coupled receptor-induced protein 2; GIG-2; phosphoprotein regulated by mitogenic pathways; SKIP1; TRB-1; Tribbles homolog 1; tribbles-like protein 1; unnamed protein product
Gene Aliases: C8FW; GIG-2; GIG2; SKIP1; TRB-1; TRB1; TRIB1
UniProt ID: (Human) Q96RU8
Entrez Gene ID: (Human) 10221
protein kinase activity
protein kinase inhibitor activity
protein binding
ATP binding
enzyme binding
protein kinase binding
enzyme regulator activity
mitogen-activated protein kinase kinase binding
ubiquitin protein ligase binding
ubiquitin-protein transferase regulator activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
transcription regulator inhibitor activity
negative regulation of transcription from RNA polymerase II promoter
negative regulation of smooth muscle cell migration
regulation of protein modification process
negative regulation of lipopolysaccharide-mediated signaling pathway
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
response to lipopolysaccharide
regulation of MAP kinase activity
negative regulation of MAPK cascade
positive regulation of eosinophil differentiation
positive regulation of macrophage differentiation
negative regulation of neutrophil differentiation
negative regulation of JNK cascade
negative regulation of smooth muscle cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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