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Product Details
PA5-97239
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | View 1 publication 1 publication |
Immunocytochemistry (ICC/IF) |
1:50-1:200 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse |
Published species |
Not Applicable |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human USP19. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
2.19 mg/mL |
Purification |
Affinity Chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.01% thimerosal |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2809041 |
Product Specific Information
Immunogen sequence: MSGGASATGP RRGPPGLEDT TSKKKQKDRA NQESKDGDPR KETGSRYVAQ AGLEPLASGD PSASASHAAG ITGSRHRTRL FFPSSSGSAS TPQEEQTKEG ACEDPHDLLA TPTPELLLDW RQSAEEVIVK LRVGVGPLQL EDVDAAFTDT DCVVRFAGGQ QWGGVFYAEI KSSCAKVQTR KGSLLHLTLP KKVPMLTWPS; Positive Samples: U-87MG, A-431, HeLa; Cellular Location: Endoplasmic reticulum membrane, Single-pass membrane protein
Target Information
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs), 1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Deubiquitinating enzyme 19; Ubiquitin carboxyl-terminal hydrolase 19; ubiquitin specific protease 19; Ubiquitin thioesterase 19; ubiquitin thiolesterase 19; ubiquitin-specific processing protease 19; Ubiquitin-specific-processing protease 19; Zinc finger MYND domain-containing protein 9
Gene Aliases: 8430421I07Rik; AI047774; KIAA0891; USP19; ZMYND9
UniProt ID: (Human) O94966, (Mouse) Q3UJD6
Entrez Gene ID: (Human) 10869, (Mouse) 71472
Molecular Function:
cysteine protease
protein deubiquitination
ER-associated ubiquitin-dependent protein catabolic process
regulation of protein stability
response to endoplasmic reticulum stress
negative regulation of skeletal muscle tissue development
protein stabilization
protein K48-linked deubiquitination
positive regulation of cell cycle process
regulation of cellular response to hypoxia
negative regulation of proteasomal protein catabolic process
regulation of ERAD pathway
proteolysis
ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
thiol-dependent ubiquitin-specific protease activity
protein binding
ubiquitin protein ligase binding
metal ion binding
Hsp90 protein binding
Lys48-specific deubiquitinase activity
cysteine-type endopeptidase activity
peptidase activity
cysteine-type peptidase activity
hydrolase activity
thiol-dependent ubiquitinyl hydrolase activity
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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