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Invitrogen
eIF3a Polyclonal Antibody
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FIGURE: 1 / 6
eIF3a Antibody (PA5-17212) in ICC/IF
Immunofluorescence analysis of eIF3a was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with eIF3a Rabbit Polyclonal Antibody(Product # PA5-17212) at 1:50 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # ... View More
Product Details
PA5-17212
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a sequence of human eIF3A
Conjugate
Form
Liquid
Concentration
15.2 µg/mL
Amount
1.52 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 150/170; Centrosomin; centrosomin homolog; cytoplasmic protein p167; eIF-3-theta; eIF-3-theta {ECO:0000255|HAMAP-Rule:MF_03000}; eIF3 p167; eIF3 p180; eIF3 p185; EIF3, p180 subunit; eIF3a; eIF3a {ECO:0000255|HAMAP-Rule:MF_03000}; Eukaryotic translation initiation factor 3 subunit 10; EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 10 (EIF-3 THETA) (EIF3 P167) (EIF3 P180) (EIF3 P185) (P162 PROTEIN) (CENTROSOMIN); eukaryotic translation initiation factor 3 subunit 10 {ECO:0000255|HAMAP-Rule:MF_03000}; Eukaryotic translation initiation factor 3 subunit A; eukaryotic translation initiation factor 3 subunit A {ECO:0000255|HAMAP-Rule:MF_03000}; eukaryotic translation initiation factor 3, subunit 10 (theta); eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD); eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD); eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa; eukaryotic translation initiation factor 3, subunit 10, 170kD; KIAA0139; p162; unnamed protein product
Gene Aliases: A830012B05Rik; Csma; EIF3; eIF3-p170; eIF3-theta; EIF3A; EIF3S10; KIAA0139; mKIAA0139; P167; p180; p185; TIF32; ZH12
UniProt ID: (Human) Q14152, (Mouse) P23116, (Rat) Q1JU68
Entrez Gene ID: (Human) 8661, (Mouse) 13669, (Rat) 292148
formation of cytoplasmic translation initiation complex
cytoplasmic translational initiation
translation reinitiation
translation
translational initiation
negative regulation of ERK1 and ERK2 cascade
IRES-dependent viral translational initiation
viral translational termination-reinitiation
formation of translation preinitiation complex
regulation of translational initiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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