Endoplasmic Reticulum (ER) Structure

The network of folded membranes that comprises the endoplasmic reticulum (ER) in all eukaryotic cells constitutes greater than half of the total membrane content in the average animal cell. The branching tubules and flattened sacs of the ER come in two forms: membrane bound by ribosomes (rough ER) or unbound by ribosomes (smooth ER). The ER has a central role in lipid and protein synthesis, protein chaperoning and folding, and calcium homeostasis. Three distinct Molecular Probes® technologies are routinely used for ER imaging in live or fixed cells.

CellLight® reagents for ER

Use CellLight® reagents when you want to label the ER in live cells to follow the dynamics of cellular behavior. CellLight® proteins can be multiplexed with other fluorescent probes for analysis in live cells or after fixation for colocalization studies. These ready-to-use constructs express fluorescent proteins with ER-retention signals from calreticulin (1.8 kDa, N-term), a resident protein of the ER lumen, and KDEL (0.5 kDa, C-term). Introducing CellLight® reagents involves a simple transfection step, and they work like cell stains with minimal toxicity or chemical disruption.

ER-Tracker™ dyes for live cells

Conjugates for fixed-cell staining

Selection guides
 
Readout
 Expression of fluorescent fusion protein
Target
 Protein retained in ER lumen
Common filter set
FITC
TRITC
Labels
GFP
RFP
Ex/Em (nm)
488/520
555/584
Signal-to-noise ratio
Photostability
Multiplexing
Yes
Yes
Live cells
Yes
Yes
Fixed cells
No
No
Fixable
Yes
Yes
Platform
 Imaging
 Imaging
Format
1 mL
1 mL
Cat. No.
 
Readout
 Live cell ER staining
Target
 Binds to K+ channels in ER
Common filter set
FITC
Texas Red®
Labels
BODIPY® FL
BODIPY® TR
Ex/Em (nm)
504/511
587/615
Signal-to-noise ratio
Photostability
Multiplexing
Yes
Yes
Live cells
Yes
Yes
Fixed cells
No
No
Fixable
Yes
Yes
Platform
 Imaging
Imaging 
Format
100 μg
100 μg
Cat. No.
 
Readout
 Fixed-cell ER staining
Target
ER-associated PDI
α-glucopyranosyl residues in ER
Common filter set
Texas Red®
FITC
Labels
Alexa Fluor® 594
Alexa Fluor® 488
Ex/Em (nm)
561/594
496/519
Signal-to-noise ratio
Photostability
Multiplexing
Yes
Yes
Live cells
No
No
Fixed cells
Yes
Yes
Fixable
No
No
Platform
Imaging
Imaging
Format
5 mg
1 set
Cat. No.
 
Readout
 Expression of fluorescent fusion protein
Target
 Protein retained in ER lumen
Common filter set
FITC
TRITC
Labels
GFP
RFP
Ex/Em (nm)
488/520
555/584
Signal-to-noise ratio
Photostability
Multiplexing
Yes
Yes
Live cells
Yes
Yes
Fixed cells
No
No
Fixable
Yes
Yes
Platform
 Imaging
 Imaging
Format
1 mL
1 mL
Cat. No.
 
Readout
 Live cell ER staining
Target
 Binds to K+ channels in ER
Common filter set
FITC
Texas Red®
Labels
BODIPY® FL
BODIPY® TR
Ex/Em (nm)
504/511
587/615
Signal-to-noise ratio
Photostability
Multiplexing
Yes
Yes
Live cells
Yes
Yes
Fixed cells
No
No
Fixable
Yes
Yes
Platform
 Imaging
Imaging 
Format
100 μg
100 μg
Cat. No.
 
Readout
 Fixed-cell ER staining
Target
ER-associated PDI
α-glucopyranosyl residues in ER
Common filter set
Texas Red®
FITC
Labels
Alexa Fluor® 594
Alexa Fluor® 488
Ex/Em (nm)
561/594
496/519
Signal-to-noise ratio
Photostability
Multiplexing
Yes
Yes
Live cells
No
No
Fixed cells
Yes
Yes
Fixable
No
No
Platform
Imaging
Imaging
Format
5 mg
1 set
Cat. No.
For Research Use Only. Not for use in diagnostic procedures.