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Invitrogen
Rat Fas ELISA Kit
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Product Specifications
Analytical sensitivity
40 pg/mL
Assay range
40-10,000 pg/mL
Sample type/volume
Plasma
50 µL
Supernatant
50 µL
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Protein name
FAS (CD95)
Species (tested)
Rat
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Rat Fas quantitates rat Fas in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant rat Fas.
Target information
CD95, also known as Fas or APO-1, is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor in the tumor necrosis factor receptor (TNFR) superfamily. It is expressed by a wide range of hematopoietic and non-hematopoietic cells, including monocytes, neutrophils, lymphocytes, and fibroblasts. CD95 also exists in a soluble form weighing approximately 26 kDa. The interaction of CD95 with its ligand, FasL, induces apoptosis, playing a crucial role in peripheral tolerance and anti-viral immune responses. Upon stimulation, CD95 leads to the aggregation of its intracellular Fas-associated death domains (FADD), formation of the death-inducing signaling complex (DISC), and activation of caspases. This cascade results in apoptosis. CD95 is also involved in activating NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and transduces proliferative signals in normal diploid fibroblasts and T cells. There are at least eight alternatively spliced transcript variants encoding seven distinct isoforms of CD95. Isoforms lacking the transmembrane domain may negatively regulate apoptosis mediated by the full-length isoform. The Fas/Fas ligand system is implicated in several human diseases, including AIDS, hepatitis, and cancer, highlighting its importance in immune regulation and disease pathology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : FasR, Tnfrsf6
Gene ID : (Rat) 246097
Gene symbol : Fas
Protein Aliases : apo-1 antigen, Apo1 antigen, Apo-1 Fas, OTTHUMP00000020045, OTTHUMP00000020046, OTTHUMP00000020051, OTTHUMP00000059646, apoptosis-mediating surface antigen FAS, CD95 antigen, Fas (TNF receptor superfamily, member 6), Fas antigen (ATP1), Fas receptor, FASLG receptor, Tumor necrosis factor receptor superfamily, member 6
UniProt ID (Rat) Q63199
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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