Electrocompetent cells work using the electroporation process. Electrical pulses created pores that allows genetic material to permeate the bacterial membrane. Invitrogen offers a variety of electrocompetent E. coli cells to reliably clone your DNA with high efficiency. The convenient packaging formats and a variety of electroporation cuvettes will fulfill all of your electrocompetent cell transformation requirements.

Electroporation Cuvettes

electroporation cuvette

Choose the right electroporation cuvette for your strain. Available in a variety of sizes.
Learn more about Electroporation Cuvettes

Electrocompetent cells available

Cell Strain

Description Application Transformation Efficiency  
MegaX DH10B T1R Electrocomp Cells

MegaX DH10B T1R Electrocomp cells are the highest-efficiency electrocompetent cells.

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Ideal for highly demanding cloning and library construction applications >3 x 1010 cfu/μg Order Now
ElectroMAX DH10BT 1 R Cells

High efficiency cells derived from DH10Bstrain. tonA genotype for T1 and T5 phage resistance. Efficient cloning of prokaryotic and eukaryotic genomic DNA. Efficient plasmid rescue from eukaryotic genomes. >1 × 1010 cfu/μg Order Now
ElectroMAX DH10B Cells


Derived from the DH10B strain in a high efficiency electrocompetent format. Also available in bulk format. Efficient cloning of both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes. >1 × 1010 cfu/μg Order Now
ElectroMAX DH5α-E Cells Derived from the DH5α strain. Suitable for transformation by electroporation. Procedures requiring high transformation efficiencies, such as cDNA library generation or with limited input DNA. >1 × 1010 cfu/μg Order Now
One Shot TOP10  Electrocompetent Cells Genetically similar to the DH10B strain. Available in single-use One Shot format. Used for general cloning applications. Found in many TOPO cloning and expression kits. >1 × 1010 cfu/μg Order Now
TOP10 Electrocompetent Cells Genetically similar to the reliable DH10B strain. Used for general cloning applications. Found in many TOPO cloning and expression kits. >1 × 1010 cfu/μg Order Now
TOP10F’ Electrocompetent Cells Genetically similar to the reliable DH10B strain with the F’ genotype.  Used for general cloning applications.  >1 × 1010 cfu/μg Order Now
ElectroMAX DH12S Cells A derivative of DH10B are suitable for transformation by electroporation. A unique genotype to support production of exceptionally clean ssDNA. >1 × 1010 cfu/μg Order Now
ElectroMAX Stbl4 Competent Cells   Suitable for transformation by electroporation. Specifically designed for cloning unstable inserts. >5 × 109 cfu/μg Order Now

Highest-efficiency electrocompetent cells

MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available (Figure 1), with a guaranteed three-fold greater number of colonies per transformation (>3 x 1010 cfu/μg of pUC control DNA). They are ideal for highly demanding cloning and library construction applications. MegaX cells have the same genotype as the widely used DH10B™ T1R strain, including tonA to prevent T1 and T5 lytic phage infection and safeguard your valuable clones and libraries; however, they are manufactured using an improved process that results in a significantly higher transformation efficiency. Available in the bulk Electrocomp format.

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MegaX  DH10B  T1R  Electrocomp

Figure 1. MegaX  DH10B T1R Electrocomp cells consistently outperform the competition. L: E.cloni® 10G SUPREME cells (Lucigen); G:GC10™ Thunderbolt™ cells (GeneChoice);  S: ElectroTen-Blue® electroporation-competent cells (Stratagene).