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EGFR Polyclonal Antibody

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EGFR Polyclonal Antibody

Product Details

100-401-149

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:10,000
-

Immunohistochemistry (IHC)

2.5 µg/mL
-

ELISA (ELISA)

1:10,000-1:50,000
-

Immunoprecipitation (IP)

10 µL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a peptide synthesized using conventional technology.The sequence of the epitope maps to a region near the carboxy terminus which is identical in human, mouse, and rat EGFR.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

85 mg/mL

Amount

21.25 mg

Storage buffer

whole serum

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antiserum is directed against human epidermal growth factor receptor (EGFR) and is useful in determining its presence in western blotting and immunoprecipitation experiments. This antibody can detect EGFR from human, mouse and rat sources. Reactivity of this antibody with EGFR from other species is unknown.

Target Information

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 2.7.10.1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; contains extracellular domain; EC 2.7.10.1; EGFR-related peptide; Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor tyrosine kinase domain; epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); erb-b2 receptor tyrosine kinase 1; Erbb1; GIG40; kinase EGFR; membrane protein; Oncogene ERBB; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; tyrosine protein kinase; unnamed protein product; Urogastrone; waved 2

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Gene Aliases: 9030024J15Rik; ERBB; ErbB-1; ERBB1; Errb1; ERRP; HER1; mENA; NISBD2; NNCIS; PIG61; wa-2; wa2; Wa5

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UniProt ID: (Human) P00533, (Mouse) Q01279

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Entrez Gene ID: (Human) 1956, (Rat) 24329, (Mouse) 13649

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Function(s)
chromatin binding double-stranded DNA binding protein kinase activity MAP kinase kinase kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity transmembrane signaling receptor activity epidermal growth factor-activated receptor activity protein binding ATP binding kinase activity enzyme binding kinase binding protein phosphatase binding protein tyrosine kinase activator activity ubiquitin protein ligase binding identical protein binding cadherin binding epidermal growth factor binding actin filament binding ATPase binding nucleotide binding receptor binding integrin binding calmodulin binding protein kinase binding macromolecular complex binding
Process(es)
cell morphogenesis liver development embryonic placenta development positive regulation of protein phosphorylation hair follicle development translation receptor-mediated endocytosis response to oxidative stress signal transduction cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway epidermal growth factor receptor signaling pathway salivary gland morphogenesis midgut development circadian rhythm positive regulation of cell proliferation epidermis development gene expression diterpenoid metabolic process peptidyl-tyrosine phosphorylation telencephalon development cerebral cortex cell migration neuron differentiation positive regulation of cell growth lung development positive regulation of cell migration response to estradiol positive regulation of superoxide anion generation positive regulation of peptidyl-serine phosphorylation response to cobalamin response to hydroxyisoflavone response to lipid positive regulation of toll-like receptor 9 signaling pathway intracellular signal transduction ERBB2-EGFR signaling pathway negative regulation of epidermal growth factor receptor signaling pathway regulation of cell proliferation negative regulation of protein catabolic process vasodilation positive regulation of phosphorylation ovulation cycle hydrogen peroxide metabolic process xenobiotic transport negative regulation of apoptotic process positive regulation of MAPK cascade protein kinase B signaling tongue development positive regulation of DNA repair positive regulation of DNA replication positive regulation of bone resorption positive regulation of vasoconstriction negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation astrocyte activation positive regulation of fibroblast proliferation digestive tract morphogenesis positive regulation of smooth muscle cell proliferation neuron projection morphogenesis positive regulation of epithelial cell proliferation regulation of peptidyl-tyrosine phosphorylation response to calcium ion positive regulation of protein kinase B signaling positive regulation of synaptic transmission, glutamatergic positive regulation of glial cell proliferation morphogenesis of an epithelial fold eyelid development in camera-type eye response to UV-A positive regulation of mucus secretion positive regulation of ERK1 and ERK2 cascade cellular response to amino acid stimulus cellular response to mechanical stimulus cellular response to growth factor stimulus cellular response to estradiol stimulus cellular response to xenobiotic stimulus cellular response to dexamethasone stimulus positive regulation of protein kinase C signaling positive regulation of canonical Wnt signaling pathway liver regeneration cell-cell adhesion positive regulation of G1/S transition of mitotic cell cycle positive regulation of prolactin secretion positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of protein localization to plasma membrane negative regulation of cardiocyte differentiation learning or memory negative regulation of macroautophagy cellular response to epidermal growth factor stimulus positive regulation of cGAS/STING signaling pathway positive regulation of protein localization to early endosome MAPK cascade ossification protein insertion into membrane
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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