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Invitrogen

IL-23 p19 Monoclonal Antibody (23dcdp), PE, eBioscience™

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View all (44) IL-23 p19 antibodies

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Product Details

12-7823-41

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test
View 4 publications 4 publications
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

23dcdp

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10669562

Product Specific Information

Description: This 23dcdp monoclonal antibody reacts with the p19 subunit of human IL-23. This heterodimeric cytokine is composed of two disulfide-linked subunits, p40 and p19. It is closely related to IL-12, with which it shares the p40 subunit. The IL-23 receptor is also heterodimeric and shares the IL-12Rbeta1 chain with IL-12, while the IL-23R chain is unique to the IL-23 receptor complex. IL-23R signaling occurs through the Jak/STAT pathway and results in RORgammat expression, which promotes maintenance and proliferation of T helper 17 (Th17) cells.

Dendritic cells and macrophages produce IL-23 in response to TLR2, TLR4, and TLR8 ligands, as well as agonists of the beta-glucan receptor, Dectin-1. Our studies suggest donor, kinetics and type of stimulant can result in variation in IL-23 expression levels. Recent publications suggest the p19 subunit may also exist in the absence of association with p40.

Applications Reported: This 23dcdp antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 23dcdp antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: il 23; IL 23 A; Il-12b; Il-12p40; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12B; Il12p40; il23; ILN; Interleukin; interleukin 12B; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; MGC79388; p40; RP23-388G23.1

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Gene Aliases: IL-23; IL-23A; IL23A; IL23P19; P19; SGRF; UNQ2498/PRO5798

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UniProt ID: (Human) Q9NPF7

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Entrez Gene ID: (Human) 51561

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Function(s)
cytokine activity protein binding interleukin-23 receptor binding
Process(es)
positive regulation of T cell mediated cytotoxicity positive regulation of defense response to virus by host immune system process positive regulation of T-helper 1 type immune response inflammatory response immune response JAK-STAT cascade regulation of gene expression positive regulation of macromolecule biosynthetic process negative regulation of interleukin-10 production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-17 production positive regulation of tumor necrosis factor production positive regulation of natural killer cell activation positive regulation of natural killer cell proliferation positive regulation of tissue remodeling T cell proliferation positive regulation of T cell proliferation positive regulation of activated T cell proliferation positive regulation of memory T cell differentiation innate immune response positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of alpha-beta T cell activation tissue remodeling positive regulation of inflammatory response defense response to Gram-negative bacterium positive regulation of NK T cell activation positive regulation of NK T cell proliferation defense response to virus regulation of primary metabolic process positive regulation of neutrophil chemotaxis receptor signaling pathway via STAT positive regulation of NIK/NF-kappaB signaling positive regulation of leukocyte differentiation positive regulation of T-helper 17 type immune response positive regulation of T-helper 17 cell lineage commitment
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