Neurobasal™ Medium, minus phenol red This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  12348017

Gibco™  Related applications: Mammalian Cell Culture | Primary Cell Culture | Stem Cell Culture

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Neurobasal® Medium Minus Phenol Red is a basal media that meets the special requirements of pre-natal and embryonic neuronal cells when used with GIBCO® B-27® Supplement, but without Phenol Red. Neurobasal® Medium can be used to grow neuronal cells from hippocampus, cortex and other regions of the brain. Neurobasal® Medium allows for both long and short term maintenance of homogeneous populations of neuronal cells without the need of an astrocyte feeder layer.

Use Neurobasal® Medium Minus Phenol Red for applications in which the presence of Phenol Red will interfere with your results.

Add Your Own Supplements
All Neurobasal® media contain no L-glutamine, L-glutamic acid, or aspartic acid. Neurobasal® media must be supplemented with 0.5 mM L-glutamine or GlutaMAX™-I Supplement and either a serum-free supplement (such as B-27® Supplement or N-2 Supplement), or with serum. For initial plating, you should add 25 µM glutamic acid.

Quality Control Testing
All Neurobasal® media are tested for pH, osmolality, endotoxin, and the absence of bacterial and fungal contamination.

We also provide Neurobasal® Medium with Phenol Red.

Neurobasal® Medium is optimized for pre-natal and embryonic neurons. For growing post-natal and adult brain neurons, we provide Neurobasal®-A Medium

See the entire Neurobasal® product line.

Learn more about neural cell culture products from Invitrogen at

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


Cell Type: Neuronal Cells (Pre-Natal), Stem Cells (Neural)
Amino Acids: No Aspartic Acid, No Glutamic Acid
Classification: Serum-Free
Culture Type: Feeder Cell-Free
Form: Liquid
Glutamine: No Glutamine
Phenol Red Indicator: No Phenol Red
Product Size: 500 mL
Serum Level: Serum-Free
Green Features: Sustainable packaging
Shipping Condition: Room Temperature

Contents & storage

Store in refrigerator (2–8°C) and protect from light.


Manuals & protocols

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