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Invitrogen
Ly-49C/I/F/H Monoclonal Antibody (14B11), Biotin, eBioscience™
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FIGURE: 1 / 1
Ly-49C/I/F/H Antibody (13-5991-82) in Flow
C57BL/6 mouse splenocytes were stained with CD49b Monoclonal Antibody, APC (Product # 17-5971-82) and 0.125 µg of Armenian Hamster IgG Isotype Control, Biotin (Product # 13-4888-85) (left) or 0.125 µg of Ly-49C/I/F/H Monoclonal Antibody, Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
13-5991-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Syrian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
14B11
Conjugate
Biotin
Biotin
Biotin
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 14B11 monoclonal antibody reacts with a subset of mouse Ly-49 receptors and recognizes a majority of mouse NK cells. This antibody reacts with 3 putative inhibitory members of the Ly-49 family, F, I, and C and one putative activating receptor, Ly-49H. 14B11 does not react with Ly-49A, B, D, or G. It is reported that 14B11 enhances the redirected lysis of FcR+ target cells by IL-2-activated NK cells.
Applications Reported: The 14B11 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The 14B11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
KLRA3 (also known as Ly49C) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA3 is thought to be an inhibitory receptor, recognizing peptide -receptive H-2Kb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cytomegalovirus resistance 1; ly-49h; lymphocyte antigen 49c; lymphocyte antigen 49f; lymphocyte antigen 49h; natural killer cell-associated antigen 2; T-cell surface glycoprotein Ly-49C; T-cell surface glycoprotein Ly-49F; T-cell surface glycoprotein Ly-49H; unnamed protein product
Gene Aliases: 5E6; Cmv-1; Cmv1; ly-49f; Ly49c; Ly49f; Ly49h; Ly49u<129>; Nk-2; NK-2.1; Nk2; Nk2.1
UniProt ID: (Mouse) Q64329, (Mouse) Q60653, (Mouse) Q60682
Entrez Gene ID: (Mouse) 16634, (Mouse) 16637, (Mouse) 16639
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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