Search

  • お問い合わせ一覧​
  • クイックオーダー

Invitrogen

IL-12/IL-23 p40 Monoclonal Antibody (C17.8), Biotin, eBioscience™

5 Published Figures
17 References
View all (40) IL-12/IL-23 p40 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:

{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}

Product Details

13-7123-81

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

-
View 3 publications 3 publications

ELISA (ELISA)

0.5-2 µg/mL
View 12 publications 12 publications

Neutralization (Neu)

-
View 1 publication 1 publication

Functional Assay (Functional)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

C17.8

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Amount

50 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466928

Product Specific Information

Description: The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells.

Applications Reported: The C17.8 antibody has been reported for use in IP, Western, ELISA, neutralization, and intracellular staining for flow cytometric analysis.

Applications Tested: The biotinylated C17.8 antibody has been tested as the detection antibody in three different sandwich ELISAs, for measurement of mouse IL-12 p70, mouse IL-23, or mouse IL-12 p40 total.

For mouse IL-12 p70 ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in sandwich ELISA for analysis of mouse IL-12 p70, in combination with the purified C18.2 antibody (14-7122) for capture and recombinant mouse IL-12 p70 (39-8121) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2,000 pg/mL - 15 pg/mL should be included in each ELISA plate. Super AquaBlue or ABTS are suitable substrates.

For mouse IL-23 ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-23 protein levels, in combination with the purified G23-8 antibody (14-7232) for capture and recombinant mouse IL-23 (39-8231) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.25 - 1 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4,000 pg/mL - 30 pg/mL should be included in each ELISA plate. Note: TMB (00-4201), rather than ABTS, should be used as the substrate.

For mouse IL-12 p40 Total ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse total p40 (e.g., p40 monomer, p40 dimer, p40/p35, and p40/p19), in combination with the purified C15.6 antibody (14-7125) for capture and recombinant mouse IL-12 p40 (39-8122), IL-12 p70 (39-8121) or mouse IL-23 (39-8231) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2,000 pg/mL - 15 pg/mL should be included in each ELISA plate. Super AquaBlue or ABTS are suitable substrates.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CLMF p35; CLMF p40; cytotoxic lymphocyte maturation factor 35 kDa subunit; cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; IL-12 p35 subunit; IL-12 p40 subunit; IL-12 subunit p35; IL-12 subunit p40; IL-23 subunit alpha; IL-23 subunit p40; IL-23-A; IL-23p19; IL12 p40; IL23; il23; IL23 p41; ILN; Interleukin; Interleukin 12; interleukin 12a p35 subunit; interleukin-12 p35 subunit; interleukin-12 p40 subunit; interleukin-23 subunit p19; Interleukin12; RP23-388G23.1; unnamed protein product

View more View less

Gene Aliases: Il-12a; Il-12b; IL-12p35; Il-12p40; IL-23; Il12p40; Ll12a; p19; p35; p40

View more View less

UniProt ID: (Mouse) P43431, (Mouse) P43432, (Mouse) Q9EQ14

View more View less

Entrez Gene ID: (Mouse) 16159, (Mouse) 16160, (Mouse) 83430

View more View less

Function(s)
cytokine activity interleukin-12 receptor binding protein binding growth factor activity interleukin-12 beta subunit binding interleukin-27 binding protein heterodimerization activity cytokine receptor activity cytokine receptor binding interleukin-12 alpha subunit binding identical protein binding macromolecular complex binding interleukin-23 receptor binding
Process(es)
positive regulation of T cell mediated cytotoxicity positive regulation of defense response to virus by host positive regulation of T-helper 1 type immune response immune response JAK-STAT cascade regulation of gene expression negative regulation of interleukin-10 production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-17 production positive regulation of tumor necrosis factor production positive regulation of natural killer cell proliferation interleukin-23-mediated signaling pathway T cell proliferation positive regulation of T cell proliferation positive regulation of activated T cell proliferation positive regulation of memory T cell differentiation positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade positive regulation of alpha-beta T cell activation defense response to Gram-negative bacterium positive regulation of NK T cell activation positive regulation of NK T cell proliferation positive regulation of neutrophil chemotaxis receptor signaling pathway via STAT positive regulation of leukocyte differentiation positive regulation of T-helper 17 type immune response positive regulation of T-helper 17 cell lineage commitment positive regulation of leukocyte mediated cytotoxicity natural killer cell activation involved in immune response positive regulation of lymphocyte mediated immunity positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target negative regulation of inflammatory response to antigenic stimulus cell surface receptor signaling pathway cell proliferation response to UV-B cell migration natural killer cell activation negative regulation of interleukin-17 production positive regulation of natural killer cell activation positive regulation of mononuclear cell proliferation positive regulation of smooth muscle cell apoptotic process interleukin-12-mediated signaling pathway T-helper cell differentiation defense response to protozoan T-helper 1 cell differentiation positive regulation of cell adhesion negative regulation of smooth muscle cell proliferation positive regulation of lymphocyte proliferation negative regulation of protein secretion regulation of inflammatory response defense response to virus cellular response to lipopolysaccharide cellular response to interferon-gamma negative regulation of vascular endothelial growth factor signaling pathway negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis response to virus response to lipopolysaccharide T-helper 1 cell activation positive regulation of tyrosine phosphorylation of STAT protein positive regulation of T cell differentiation positive regulation of natural killer cell mediated cytotoxicity defense response to Gram-positive bacterium extrinsic apoptotic signaling pathway cellular response to virus positive regulation of dendritic cell chemotaxis
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support