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CD278 (ICOS) Monoclonal Antibody (7E.17G9), Biotin, eBioscience™
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FIGURE: 1 / 7
CD278 (ICOS) Antibody (13-9942-85) in Flow
Staining of unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.03 µg of Rat IgG2b Isotype Control Biotin (Product # 13-4031-82) (open histogram) or 0.03 µg of Anti-Mouse CD278 (ICOS) Biotin followed by Streptavidin PE (Product # 12-4317-87). Viable cells were analyzed.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
13-9942-85
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
7E.17G9
Conjugate
Biotin
Biotin
Biotin
Form
Liquid
Concentration
0.5 mg/mL
Amount
500 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.
Applications Reported: 7E.17G9 has been reported for use in flow cytometric analysis.
Applications Tested: The 7E.17G9 antibody has been tested by flow cytometric analysis of unstimulated and Con A-stimulated (3 day) mouse splenocytes and can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ICOS (CD278) encodes a protein that is a member of the CD28 and CTLA-4 cell-surface receptor family. It functions as a homodimer and has a crucial role in cell-cell signaling, immune responses, and the regulation of cell proliferation. ICOS has been linked to various disorders, including Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: activation-inducible lymphocyte immunomediatory molecule; CD28 and CTLA-4 like protein; CD28 and CTLA-4-like protein; CD28-related protein 1; CRP-1; unnamed protein product
Gene Aliases: AILIM; CCLP; CRP-1; H4; Ly115
UniProt ID: (Mouse) Q9WVS0
Entrez Gene ID: (Mouse) 54167
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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