Search
Invitrogen
CD140a (PDGFRA) Monoclonal Antibody (APA5), Functional Grade, eBioscience™
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 91
CD140a (PDGFRA) Antibody (16-1401-82) in Flow
Staining of NIH/3T3 cells with 0.5 µg of Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.5 µg of Anti-Mouse CD140a (PDGF Receptor a) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
16-1401-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse,
Rat
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
APA5
Conjugate
Functional Grade
Functional Grade
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.
Applications Reported: This APA5 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistology staining of frozen tissue sections. It has also been reported for blocking ligand binding.
Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of the NIH/3T3 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Alpha platelet-derived growth factor receptor precursor (PDGF-R-alpha); alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF alpha chain; PDGF Receptor alpha; PDGF subunit A; PDGF-1; PDGF-R-alpha; pdgfr-a; PDGFR-alpha; unnamed protein product
Gene Aliases: CD140a; Pdgfr-2
UniProt ID: (Mouse) P26618
Entrez Gene ID: (Mouse) 18595
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
platelet-derived growth factor alpha-receptor activity
vascular endothelial growth factor-activated receptor activity
platelet-derived growth factor receptor binding
ATP binding
vascular endothelial growth factor binding
protein homodimerization activity
phosphatidylinositol 3-kinase binding
macromolecular complex binding
platelet-derived growth factor binding
phospholipase C activator activity
luteinization
in utero embryonic development
hematopoietic progenitor cell differentiation
response to stress
transmembrane receptor protein tyrosine kinase signaling pathway
estrogen metabolic process
positive regulation of cell proliferation
male gonad development
female gonad development
anatomical structure morphogenesis
organ morphogenesis
negative regulation of platelet activation
cell migration
peptidyl-tyrosine phosphorylation
signal transduction involved in regulation of gene expression
extracellular matrix organization
lung development
adrenal gland development
positive regulation of cell migration
male genitalia development
Leydig cell differentiation
cellular response to reactive oxygen species
platelet-derived growth factor receptor-alpha signaling pathway
positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway
wound healing
odontogenesis of dentin-containing tooth
protein autophosphorylation
platelet-derived growth factor receptor signaling pathway
positive regulation of fibroblast proliferation
embryonic digestive tract morphogenesis
embryonic cranial skeleton morphogenesis
skeletal system morphogenesis
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of calcium-mediated signaling
white fat cell differentiation
positive regulation of chemotaxis
cardiac myofibril assembly
palate development
face morphogenesis
cell chemotaxis
lung growth
positive regulation of branching involved in lung morphogenesis
retina vasculature development in camera-type eye
positive regulation of ERK1 and ERK2 cascade
platelet aggregation
cellular response to amino acid stimulus
metanephric glomerular capillary formation
regulation of mesenchymal stem cell differentiation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support