Search
Invitrogen
Axl Monoclonal Antibody (DS7HAXL), APC, eBioscience™
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
{{$ctrl.translations['antibody.pdp.skuNotes.additionalInformation'] || 'Additional Information'}}:
{{$ctrl.translations['antibody.pdp.skuNotes.additionalInformation']}}:
FIGURE: 1 / 6
Axl Antibody (17-1087-42) in Flow
Normal human peripheral blood cells were stained with HLA-DR Monoclonal Antibody, FITC (Product # 11-9956-42), CD1c Monoclonal Antibody, PE (Product # 12-0015-42), CD11c Monoclonal Antibody, eFluor 450 (Product # 49-0116-42), and Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-82) (left) or Axl Monoclonal Antibody, APC (right). CD11c+/CD14- cells were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
17-1087-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
DS7HAXL
Conjugate
APC
APC
APC
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This DS7HAXL monoclonal antibody recognizes human Axl, a member of the TAM family of tyrosine kinase receptors that also includes MerTK and Tyro3. This DS7HAXL antibody will work in flow cytometry on both native and paraformaldehyde-fixed cells. It has also been shown to stain formalin-fixed, paraffin-embedded tissues for IHC analysis.
Applications Reported: This DS7HAXL antibody has been reported for use in flow cytometric analysis.
Applications Tested: This DS7HAXL antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
Target Information
AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: AXL oncogene; AXL transforming sequence/gene; AZF1; oncogene AXL; putative tyrosine kinase receptor; sAXL; soluble AXL; unnamed protein product
Gene Aliases: ARK; AXL3; JTK11; Tyro7; UFO
UniProt ID: (Human) P30530
Entrez Gene ID: (Human) 558
natural killer cell differentiation
negative regulation of cytokine production
positive regulation of cytokine-mediated signaling pathway
phagocytosis
signal transduction
enzyme linked receptor protein signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
nervous system development
positive regulation of signal transduction
cell migration
platelet activation
negative regulation of interferon-gamma production
positive regulation of natural killer cell differentiation
cellular response to interferon-alpha
negative regulation of apoptotic process
viral entry into host cell
vascular endothelial growth factor receptor signaling pathway
cell maturation
positive regulation of pinocytosis
regulation of lymphocyte activation
positive regulation of protein kinase B signaling
cellular response to lipopolysaccharide
dendritic cell differentiation
mononuclear cell differentiation
positive regulation of viral life cycle
negative regulation of dendritic cell apoptotic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support