Thermo Scientific™

Clean-Blot™ IP Detection Reagent (HRP)

Catalog number:  21230

 Related applications:

Protein Electrophoresis & Western Blotting

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Thermo Scientific Clean-Blot IP Detection Reagent is a horseradish peroxidase conjugate that is optimized for post-immunoprecipitation Western blot detection of primary antibodies without interference from denatured IP antibody fragments.

The Clean-Blot IP Reagent allows trouble-free Western blot detection of target proteins following IP assays (immunoprecipitation). It works by specifically binding to functional primary antibodies (whole IgG) without also binding to fragments of the IP antibodies, which usually accompany the immunoprecipitated protein through electrophoresis and membrane transfer. The Clean-Blot IP Reagent and Kit eliminate detection-interference from both heavy-chain (approx. 50kDa) and light-chain (25kDa) IgG-fragments of antibodies used for the initial immunoprecipitation assay.

Features of the Clean-Blot IP Detection Reagent:

Universal—bind and detect most species, IgG subclasses and isotypes of primary antibodies that are commonly used for Western blotting
Compatible—effective with IP assays performed using Protein A, Protein G, or anti-IgG agarose beads and any blocking buffer
Cost-effective—eliminates the cost and extra work associated with covalently immobilizing IP antibodies as a means of overcoming Western blot interference
Flexible—horseradish peroxidase (HRP) reagent for detection with chemiluminescent, fluorescent or colorimetric substrates
Easy to use—no need to change the Western blotting protocol; simply replace conventional secondary HRP conjugate with the Clean-Blot IP Detection Reagent
Unobstructed detection—clear Western blot results for immmunoprecipitation assays without significant interference from denatured IgG bands

Related Products
Clean-Blot™ IP Detection Kit (HRP)
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Western Blot
Product Line: Pierce™
Product Size: 2.5 mL
Reactivity: All species

Contents & storage

Store at 4°C.


Manuals & protocols