Clean-Blot™ IP Detection Kit (HRP)

Catalog number: 21232

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Thermo Scientific Clean-Blot IP Detection Kit (HRP) contains enough Clean-Blot IP Detection Reagent and accessory reagents for 25 mini-blots or 2000 cm2 of membrane. Clean-Blot IP Detection Reagent is a horseradish peroxidase conjugate that is optimized for post-immunoprecipitation Western blot detection of primary antibodies without interference from denatured IP antibody fragments.

The Clean-Blot IP Reagent allows trouble-free Western blot detection of target proteins following IP assays (immunoprecipitation). It works by specifically binding to functional primary antibodies (whole IgG) without also binding to fragments of the IP antibodies, which usually accompany the immunoprecipitated protein through electrophoresis and membrane transfer. The Clean-Blot IP Reagent and Kit eliminate detection-interference from both heavy-chain (approx. 50kDa) and light-chain (25kDa) IgG-fragments of antibodies used for the initial immunoprecipitation assay.

Features of the Clean-Blot IP Detection Reagent:

Universal—bind and detect most species, IgG subclasses and isotypes of primary antibodies that are commonly used for Western blotting
Compatible—effective with IP assays performed using Protein A, Protein G, or anti-IgG agarose beads and any blocking buffer
Cost-effective—eliminates the cost and extra work associated with covalently immobilizing IP antibodies as a means of overcoming Western blot interference
Flexible—horseradish peroxidase (HRP) reagent for detection with chemiluminescent, fluorescent or colorimetric substrates
Easy to use—no need to change the Western blotting protocol; simply replace conventional secondary HRP conjugate with the Clean-Blot IP Detection Reagent
Unobstructed detection—clear Western blot results for immmunoprecipitation assays without significant interference from denatured IgG bands

Included in this kit:
Kit contains Clean-Blot Detection Reagent (HRP), blocking buffer and Thermo Scientific Pierce ECL Substrate (chemiluminescent)

Related Products
Clean-Blot™ IP Detection Reagent (HRP)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reactivity: All species
Product Size: 25 blots
Detection Method: Western Blot

Contents & storage

Sufficient For: 25 mini blots or 2000 cm2 membrane
• Clean-Blot Detection Reagent (HRP), 2.5 mL
• StartingBlock T20 (TBS) Blocking Buffer, 1 L
• Pierce ECL Detection Reagent 1, Peroxide Solution, 125 mL
• Pierce ECL Detection Reagent 2, Luminol Enhancer Solution, 125 mL

Store at 4°C.

Documents

Manuals & protocols