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          • Primary Antibodies ›
          • AMPK alpha-1,2 Antibodies

          Invitrogen

          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody

          Advanced Verification
          4 Published Figures
          11 References
          View all (14) AMPK alpha-1,2 antibodies

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          Cite Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          • Published Figures (4)
          • Advanced Verification (1)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Immunocytochemistry (ICC/IF)
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          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody (44-1150G) in ICC/IF

          Immunofluorescence analysis of Phospho-AMPK alpha 1,2 (Thr183, Thr172) was done on 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-AMPK alpha-1,2 (Thr183, Thr172) Rabbit Polyclonal Antibody (Product # 44-115... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody in Western Blot (WB)
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody
          Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody

          Product Details

          44-1150G

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 5 publications 5 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:10-1:50
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:250
          View 2 publications 2 publications

          Flow Cytometry (Flow)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human AMPK alpha 1 that contains threonine 183 and AMPK alpha 2 that contains threonine 172. The sequence is conserved in human, mouse and rat.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Purification

          Antigen affinity chromatography

          Storage buffer

          Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533585

          Target Information

          The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase alpha-2 variant B; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 2; AMPK alpha-2 variant 2; AMPK alpha-2 variant 3; AMPK subunit alpha-1; AMPK subunit alpha-2; AMPK, alpha, 1; AMPK-alpha-1 chain; AMPK-alpha-2 chain; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; pAMPK; prkaa2; protein kinase; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, alpha 2 catalytic subunit; Tau-protein kinase PRKAA1; unnamed protein product

          View more View less

          Gene Aliases: 2310008I11Rik; A830082D05; AI194361; AI450832; AL024255; AMPK; AMPK alpha 1; AMPK1; AMPK2; AMPKa1; AMPKa2; AMPKalpha1; AMPKalpha2; C130083N04Rik; PRKAA; PRKAA1; PRKAA2

          View more View less

          UniProt ID: (Human) Q13131, (Human) P54646, (Mouse) Q5EG47, (Mouse) Q8BRK8

          View more View less

          Entrez Gene ID: (Human) 5562, (Human) 5563, (Mouse) 105787, (Mouse) 108079

          View more View less

          Function(s)
          nucleotide binding chromatin binding protein kinase activity protein serine/threonine kinase activity AMP-activated protein kinase activity cAMP-dependent protein kinase activity protein binding ATP binding kinase activity transferase activity macromolecular complex binding metal ion binding [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity tau protein binding tau-protein kinase activity protein serine kinase activity histone H2BS36 kinase activity protein serine/threonine/tyrosine kinase activity protein C-terminus binding transferase activity, transferring phosphorus-containing groups kinase binding histone serine kinase activity [acetyl-CoA carboxylase] kinase activity protein binding, bridging non-receptor serine/threonine protein kinase
          Process(es)
          autophagosome assembly response to hypoxia cytoplasmic translation negative regulation of T cell mediated immune response to tumor cell positive regulation of T cell mediated immune response to tumor cell glucose metabolic process chromatin organization chromatin remodeling lipid metabolic process fatty acid metabolic process fatty acid biosynthetic process phosphatidylethanolamine biosynthetic process phosphatidylcholine biosynthetic process steroid biosynthetic process cholesterol biosynthetic process autophagy lysosome organization signal transduction steroid metabolic process cholesterol metabolic process positive regulation of cell proliferation lipid biosynthetic process cellular response to starvation response to xenobiotic stimulus response to UV cold acclimation response to gamma radiation positive regulation of autophagy positive regulation of gene expression negative regulation of gene expression response to activity Wnt signaling pathway sterol biosynthetic process fatty acid oxidation response to caffeine SCF-dependent proteasomal ubiquitin-dependent protein catabolic process protein destabilization cellular response to nutrient levels negative regulation of TOR signaling cellular response to stress cellular response to amino acid starvation cellular response to oxidative stress TORC1 signaling cellular response to glucose starvation response to hydrogen peroxide glucose homeostasis regulation of circadian rhythm negative regulation of apoptotic process regulation of vascular permeability proteasome-mediated ubiquitin-dependent protein catabolic process response to estrogen positive regulation of cholesterol biosynthetic process positive regulation of glycolytic process positive regulation of transcription, DNA-templated negative regulation of translational initiation positive regulation of translational initiation negative regulation of glucosylceramide biosynthetic process negative regulation of insulin receptor signaling pathway rhythmic process positive regulation of skeletal muscle tissue development protein stabilization negative regulation of T cell activation positive regulation of T cell activation negative regulation of lipid catabolic process fatty acid homeostasis regulation of vesicle-mediated transport protein localization to lysosome motor behavior CAMKK-AMPK signaling cascade regulation of stress granule assembly protein-containing complex assembly neuron cellular homeostasis cellular response to hydrogen peroxide regulation of microtubule cytoskeleton organization cellular response to calcium ion cellular response to glucose stimulus cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to hypoxia cellular response to xenobiotic stimulus protein K6-linked ubiquitination positive regulation of release of cytochrome c from mitochondria energy homeostasis hepatocyte apoptotic process negative regulation of protein localization to nucleus positive regulation of transcription from mitochondrial promoter positive regulation of cellular protein localization negative regulation of hepatocyte apoptotic process positive regulation of protein targeting to mitochondrion positive regulation of adipose tissue development negative regulation of TORC1 signaling positive regulation of TORC1 signaling negative regulation of tubulin deacetylation lipid droplet disassembly protein localization to lipid particle regulation of gene expression response to muscle activity positive regulation of macroautophagy regulation of macroautophagy activation of MAPK activity transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation phosphorylation chromatin modification regulation of peptidyl-serine phosphorylation histone-serine phosphorylation intracellular signal transduction protein heterooligomerization response to camptothecin regulation of energy homeostasis negative regulation of glucose import in response to insulin stimulus response to stress regulation of lipid metabolic process cellular response to drug
          It has to be done as per old AB suggested Products section.

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