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Invitrogen
CCL3 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
CCL3 Antibody (500-P38BT-25UG) in WB
Western Blot: To detect Human MIP-1alpha by Western Blot analysis CCL3 Polyclonal Antibody, Biotin (Product # 500-P38BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIP-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
500-P38BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human MIP-1alpha (CCL3)
Conjugate
Biotin
Biotin
Biotin
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-1alpha (CCL3). Anti-Human MIP-1alpha (CCL3)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human MIP-1alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MIP-1alpha (500-P38) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-1alpha.
Western Blot: To detect Human MIP-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIP-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P38BT-1MG will be provided as 2 x 500 µg
Target Information
Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: G0/G1 switch regulatory protein 19-1; G0S19-1 peptide precursor; H-MIP-1-alpha; LD78-alpha; macrophage inflammatory protein 1-alpha; mip1 alpha; PAT 464.1; RP23-320E6.7; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); tonsillar lymphocyte LD78 alpha protein
Gene Aliases: G0S19-1; LD78; LD78ALPHA; MIP-1-alpha; MIP1A; SCI; SCYA3
UniProt ID: (Human) P10147
Entrez Gene ID: (Human) 6348
MAPK cascade
osteoblast differentiation
cell activation
monocyte chemotaxis
calcium ion transport
cellular calcium ion homeostasis
exocytosis
chemotaxis
inflammatory response
immune response
cytoskeleton organization
cell-cell signaling
regulation of cell shape
response to toxic substance
positive regulation of gene expression
negative regulation of gene expression
T cell chemotaxis
release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
calcium-mediated signaling
signaling
positive regulation of cell migration
negative regulation of bone mineralization
neutrophil chemotaxis
positive regulation of interleukin-1 beta production
positive regulation of tumor necrosis factor production
eosinophil degranulation
positive regulation of neuron apoptotic process
astrocyte cell migration
negative regulation by host of viral transcription
negative regulation of osteoclast differentiation
eosinophil chemotaxis
macrophage chemotaxis
lymphocyte chemotaxis
positive regulation of inflammatory response
regulation of behavior
positive regulation of calcium-mediated signaling
positive chemotaxis
positive regulation of protein kinase B signaling
positive regulation of calcium ion transport
regulation of sensory perception of pain
cell chemotaxis
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
response to cholesterol
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
granulocyte chemotaxis
positive regulation of calcium ion import
positive regulation of microglial cell activation
positive regulation of microglial cell migration
positive regulation of natural killer cell chemotaxis
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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