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Invitrogen
WWC1 Recombinant Rabbit Monoclonal Antibody (1H4L22)
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FIGURE: 1 / 3
WWC1 Antibody (703009) in ICC/IF
Knockdown of WWC1 was achieved by transfecting Caco-2 cells with specific siRNA (Silencer® select Product # s23476, s23478). Immunofluorescence analysis was performed on Caco2 cells (untransfected, panel a-d), transfected with WWC1 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labelled with Anti-WWC1 Recombinant Rabbit Monoclonal Antibody (Product # 703009, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjuga... View More
Product Details
703009
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
1H4L22
Immunogen
Protein corresponding to human WWC1 [aa772-aa851]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Dog, Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
WWC1 (Protein KIBRA) is a probable regulator of the Hippo/SWH signaling pathway. This pathway plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. WWC1, along with NF2, can synergistically induce the phosphorylation of LATS1 and LATS2. It acts as a transcriptional coactivator of ESR1 - which plays an essential role in DYNLL1-mediated ESR1 transactivation. WWC1 also functions as a regulatior of collagen-stimulated activation of the ERK/MAPK cascade.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytosolic WW domain-containing protein; FLJ10865; FLJ23369; HBeAg-binding protein 3; HBebp36; KIBRA; Kidney and brain protein; Protein KIBRA; protein phosphatase 1, regulatory subunit 168; protein WWC1; unnamed protein product; WW domain-containing protein 1; WW, C2 and coiled-coil domain containing 1
Gene Aliases: HBEBP3; HBEBP36; KIAA0869; KIBRA; MEMRYQTL; PPP1R168; WWC1
UniProt ID: (Human) Q8IX03
Entrez Gene ID: (Human) 23286
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
negative regulation of cell proliferation
cell migration
establishment of cell polarity
hippo signaling
regulation of hippo signaling
negative regulation of hippo signaling
positive regulation of hippo signaling
positive regulation of MAPK cascade
positive regulation of transcription, DNA-templated
negative regulation of organ growth
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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