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GHSR Antibody (720278) in WB

Western blot analysis was performed on tissue and membrane enriched cell extracts (30 µg lysate) of A549 (Lane 1), U-87 MG (Lane 2), Hep G2 (Lane 3), Mouse liver (Lane 4), Rat liver (Lane 5), SH-SY5Y (Lane 6), HEL 92.1.7 (Lane 7) and NTERA-2 (Lane 8). The blots were probed with Growth hormone secretagogue receptor 1a/GHSR1a Rabbit Polyclonal antibody (Product # 720278, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dil... View More

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

GHSR Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A549 cells were transfected with GHSR siRNA and reduction of signal was observed in Western Blot using GHSR Polyclonal Antibody (Product # 720278). {KD}

Immunogen image

Immunogen Image GHSR Polyclonal Antibody

Product Details

720278

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide corresponding to Human GHSR (aa 347-366)

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2-7.4, with 30% glycerol, 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Ambient (domestic); Wet ice (international)

AB_2633233

Product Specific Information

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

This antibody is predicted to react with Monkey, Cat, Rat and Bovine.

Target Information

Growth hormone secretagogue receptor 1a (GHSR) is a member of the G-protein coupled receptor family. GHSR may play a role in energy homeostasis and regulation of body weight. Two identified transcript variants are expressed in several tissues and are evolutionary conserved in fish and swine. One transcript, 1a, excises an intron and encodes the functional protein; this protein is the receptor for the Ghrelin ligand and defines a neuroendocrine pathway for growth hormone release. The second transcript (1b) retains the intron and does not function as a receptor for Ghrelin; however, it may function to attenuate activity of isoform 1a. Mutations to the gene can cause isolated partial growth hormone deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: G-protein coupled receptor; GH-releasing peptide receptor; ghrelin receptor; GHRP; Ghrp receptor; GHS receptor; GHS-R; unnamed protein product

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Gene Aliases: C530020I22Rik; GHDP; GHRP; GHS-R; GHS-R1a; GHSR-1a; Ghsr1a

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UniProt ID: (Human) Q92847, (Mouse) Q99P50, (Rat) O08725

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Entrez Gene ID: (Human) 2693, (Mouse) 208188, (Rat) 84022

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Function(s)

growth hormone secretagogue receptor activity G-protein coupled receptor activity protein binding coreceptor activity growth hormone-releasing hormone receptor activity peptide hormone binding identical protein binding hormone binding growth hormone receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GHSR Polyclonal Antibody

GHSR Antibody (720278) in WB

Product Details

720278

Western Blot (WB)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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