Reverse phase protein microarray technology in traumatic brain injury.
AuthorsGyorgy AB,Walker J,Wingo D,Eidelman O,Pollard HB,Molnar A,Agoston DV
JournalJournal of neuroscience methods
PubMed ID20674607
Antibody based, high throughput proteomics technology represents an exciting new approach in understanding the pathobiologies of complex disorders such as cancer, stroke and traumatic brain injury. Reverse phase protein microarray (RPPA) can complement the classical methods based on mass spectrometry as a high throughput validation and quantification method. RPPA technology ... More
The concentration of brain homogenates with the Amicon Ultra Centrifugal filters.
AuthorsYang J,Sumbria RK
JournalMethodsX
PubMed ID35004217
Accurately measuring the brain concentration of a neurotherapeutic is critical in determining its pharmacokinetic profile in vivo. Biologics are potential therapeutics for neurologic diseases and biologics fused to an antibody targeting a transcytosis receptor at the Blood-Brain Barrier, designated as antibody-biologic fusion proteins, are Blood-Brain Barrier penetrating neurotherapeutics. The use ... More
Optimized Workflow for Proteomics and Phosphoproteomics With Limited Tissue Samples.
AuthorsHu M,Wang Y
JournalCurrent protocols
PubMed ID38646944
Proteomics and phosphoproteomics play crucial roles in elucidating the dynamics of post-transcriptional processes. While experimental methods and workflows have been established in this field, a persistent challenge arises when dealing with small samples containing a limited amount of protein. This limitation can significantly impact the recovery of peptides and phosphopeptides. ... More
MicroRNA-125b is a novel negative regulator of p53.
AuthorsLe MT, Teh C, Shyh-Chang N, Xie H, Zhou B, Korzh V, Lodish HF, Lim B,
JournalGenes Dev
PubMed ID19293287
The p53 transcription factor is a key tumor suppressor and a central regulator of the stress response. To ensure a robust and precise response to cellular signals, p53 gene expression must be tightly regulated from the transcriptional to the post-translational levels. Computational predictions suggest that several microRNAs are involved in ... More
Apelin is necessary for the maintenance of insulin sensitivity.
AuthorsYue P, Jin H, Aillaud M, Deng AC, Azuma J, Asagami T, Kundu RK, Reaven GM, Quertermous T, Tsao PS
JournalAm J Physiol Endocrinol Metab
PubMed ID19861585
'The recently discovered peptide apelin is known to be involved in the maintenance of insulin sensitivity. However, questions persist regarding its precise role in the chronic setting. Fasting glucose, insulin, and adiponectin levels were determined on mice with generalized deficiency of apelin (APKO). Additionally, insulin (ITT) and glucose tolerance tests ... More
Cutting edge: NLRP12 controls dendritic and myeloid cell migration to affect contact hypersensitivity.
AuthorsArthur JC, Lich JD, Ye Z, Allen IC, Gris D, Wilson JE, Schneider M, Roney KE, O'Connor BP, Moore CB, Morrison A, Sutterwala FS, Bertin J, Koller BH, Liu Z, Ting JP,
JournalJ Immunol
PubMed ID20861349
'Nucleotide-binding domain leucine-rich repeat (NLR) proteins are regulators of inflammation and immunity. Although first described 8 y ago, a physiologic role for NLRP12 has remained elusive until now. We find that murine Nlrp12, an NLR linked to atopic dermatitis and hereditary periodic fever in humans, is prominently expressed in dendritic ... More
Novel roles for osteopontin and clusterin in peripheral motor and sensory axon regeneration.
AuthorsWright MC, Mi R, Connor E, Reed N, Vyas A, Alspalter M, Coppola G, Geschwind DH, Brushart TM, Höke A,
Journal
PubMed ID24478351
'Previous studies demonstrated that Schwann cells (SCs) express distinct motor and sensory phenotypes, which impact the ability of these pathways to selectively support regenerating neurons. In the present study, unbiased microarray analysis was used to examine differential gene expression in denervated motor and sensory pathways in rats. Several genes that ... More
Lifelong running reduces oxidative stress and degenerative changes in the testes of mice.
'Regular exercise can counteract the adverse effects of aging on the musculoskeletal and cardiovascular systems. In males, the normal aging process is associated with reductions in testosterone production and impaired spermatogenesis, but the underlying mechanisms and their potential modification by exercise are unknown. Here, we report that lifelong regular exercise ... More
Distinct functions of activated protein C differentially attenuate acute kidney injury.
AuthorsGupta A, Gerlitz B, Richardson MA, Bull C, Berg DT, Syed S, Galbreath EJ, Swanson BA, Jones BE, Grinnell BW,
JournalJ Am Soc Nephrol
PubMed ID19092124
'Administration of activated protein C (APC) protects from renal dysfunction, but the underlying mechanism is unknown. APC exerts both antithrombotic and cytoprotective properties, the latter via modulation of protease-activated receptor-1 (PAR-1) signaling. We generated APC variants to study the relative importance of the two functions of APC in a model ... More
Interaction of HIF-1a and Notch3 Is Required for the Expression of Carbonic Anhydrase 9 in Breast Carcinoma Cells.
AuthorsShareef MM, Udayakumar TS, Sinha VK, Saleem SM, Griggs WW,
Journal
PubMed ID24386511
Expression of carbonic anhydrase 9 (CA9) is associated with poor prognosis and increased tumor aggressiveness and does not always correlate with HIF-1a expression. Presently, we analyzed the regulation of CA9 expression during hypoxia by HIF-1a, Notch3, and the von Hippel-Lindau (VHL) in breast carcinoma cells. Both HIF-1a and Notch3 were ... More
Iron reduction by deferoxamine leads to amelioration of adiposity via the regulation of oxidative stress and inflammation in obese and type 2 diabetes KKAy mice.
AuthorsTajima S, Ikeda Y, Sawada K, Yamano N, Horinouchi Y, Kihira Y, Ishizawa K, Izawa-Ishizawa Y, Kawazoe K, Tomita S, Minakuchi K, Tsuchiya K, Tamaki T,
JournalAm J Physiol Endocrinol Metab
PubMed ID21917632
Iron is an essential trace metal for most organisms. However, excess iron causes oxidative stress through production of highly toxic hydroxyl radicals via the Fenton/Haber-Weiss reaction. Iron storage in the body is reported to be associated with fat accumulation and type 2 diabetes mellitus. We investigated the role of iron ... More
Cardiac-specific deletion of LKB1 leads to hypertrophy and dysfunction.
AuthorsIkeda Y, Sato K, Pimentel DR, Sam F, Shaw RJ, Dyck JR, Walsh K,
JournalJ Biol Chem
PubMed ID19828446
LKB1 encodes a serine/threonine kinase, which functions upstream of the AMP-activated protein kinase (AMPK) superfamily. To clarify the role of LKB1 in heart, we generated and characterized cardiac myocyte-specific LKB1 knock-out (KO) mice using alpha-myosin heavy chain-Cre deletor strain. LKB1-KO mice displayed biatrial enlargement with atrial fibrillation and cardiac dysfunction ... More
Subcellular transport of EKLF and switch-on of murine adult beta maj globin gene transcription.
Erythroid Krüppel-like factor (EKLF) is an essential transcription factor for mammalian beta-like globin gene switching, and it specifically activates transcription of the adult beta globin gene through binding of its zinc fingers to the promoter. It has been a puzzle that in the mouse, despite its expression throughout the erythroid ... More
Laser-capture microdissection.
AuthorsEspina V, Wulfkuhle JD, Calvert VS, VanMeter A, Zhou W, Coukos G, Geho DH, Petricoin EF, Liotta LA
JournalNat Protoc
PubMed ID17406286
Deciphering the cellular and molecular interactions that drive disease within the tissue microenvironment holds promise for discovering drug targets of the future. In order to recapitulate the in vivo interactions thorough molecular analysis, one must be able to analyze specific cell populations within the context of their heterogeneous tissue microecology. ... More
Evaluation of non-formalin tissue fixation for molecular profiling studies.
AuthorsGillespie JW, Best CJ, Bichsel VE, Cole KA, Greenhut SF, Hewitt SM, Ahram M, Gathright YB, Merino MJ, Strausberg RL, Epstein JI, Hamilton SR, Gannot G, Baibakova GV, Calvert VS, Flaig MJ, Chuaqui RF, Herring JC, Pfeifer J, Petricoin EF, Linehan WM, Duray PH, Bova GS, Emmert-Buck MR
JournalAm J Pathol
PubMed ID11839565
Using a general strategy for evaluating clinical tissue specimens, we found that 70% ethanol fixation and paraffin embedding is a useful method for molecular profiling studies. Human prostate and kidney were used as test tissues. The protein content of the samples was analyzed by one-dimensional gel electrophoresis, immunoblot, two-dimensional gel ... More