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Invitrogen
Dinitrophenyl-KLH Polyclonal Antibody, Alexa Fluor™ 488
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Product Details
A-11097
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Dinitrophenyl-KLH
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
2 mg/mL
Amount
1 mg
Purification
purified
Storage buffer
PBS, pH 7.5
Contains
5mM sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The anti-DNP-KLH antibody is supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, containing 5 mM sodium azide. The degree of labeling is indicated on the product label. This antibody was raised in rabbit against DNP conjugated to keyhole limpet hemocyanin. The IgG fraction, which contains both anti-DNP and anti-KLH IgGs, was isolated from total serum and determined to be >95% pure by SDS-polyacrylamide gel electrophoresis. It is a good practice to centrifuge protein conjugate solutions briefly in a microcentrifuge before use; only the supernatant should then be added to the experiment. This step will eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. Peak absorption and fluorescence emission wavelengths for Alexa Fluor™ 488 anti-DNPKLH conjugate is 494 nm and 520 nm, respectively
Because of their high affinity for the dinitrophenyl hapten1,2 (DNP), Invitrogen's antibodies against the dinitrophenyl- keyhole limpet hemocyanin conjugate (anti-DNP-KLH) are sensitive probes for detecting DNP-labeled molecules. The fluorescent and biotinylated anti-DNP-KLH conjugates can serve as second-step reagents for signal amplification in protein and nucleic acid detection systems, including in situ hybridization studies employing DNP-modified DNA probes. Because anti-DNP antibody binds well to DAMP, an acidotropic reagent that can penetrate cell membranes, anti-DNP-KLH antibodies can also be used with DAMP for immunochemical localization of intracellular compartments with low pH. In addition, anti-DNP-KLH antibodies may recognize compounds with a trinitrophenyl group, allowing the isolation of cell-surface molecules of human melanoma cells that have been labeled with trinitrobenzene sulfonic acid (TNBS).
Target Information
DNP-KLH antibodies are used for detecting DNP-labeled molecules. Fluorescent and biotinylated anti-DNP-KLH conjugates can serve as second-step reagents for signal amplification in protein and nucleic acid detection systems, including in situ hybridization studies employing DNP-modified DNA probes. Because anti-DNP antibody binds well to DAMP (D-1552), an acidotropic reagent that can penetrate cell membranes, anti-DNP-KLH antibodies can also be used with DAMP for immunochemical localization of intracellular compartments with low pH. DNP-KLH antibodies may recognize compounds with a trinitrophenyl group, allowing the isolation of cell-surface molecules of human melanoma cells that have been labeled with trinitrobenzene sulfonic acid (TNBS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C6H3N2O4; DNP-KLH
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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