Differential requirement for classic and novel PKC isoforms in respiratory burst and phagocytosis in RAW 264.7 cells.
AuthorsLarsen EC, DiGennaro JA, Saito N, Mehta S, Loegering DJ, Mazurkiewicz JE, Lennartz MR
JournalJ Immunol
PubMed ID10946313
'The binding of Ab (IgG)-opsonized particles by FcgammaRs on macrophages results in phagocytosis of the particles and generation of a respiratory burst. Both IgG-stimulated phagocytosis and respiratory burst involve activation of protein kinase C (PKC). However, the specific PKC isoforms required for these responses have yet to be identified. We ... More
Gbetagamma activation of Src induces caveolae-mediated endocytosis in endothelial cells.
AuthorsShajahan AN, Tiruppathi C, Smrcka AV, Malik AB, Minshall RD,
JournalJ Biol Chem
PubMed ID15345719
'Caveolae-mediated endocytosis in endothelial cells is stimulated by the binding of albumin to gp60, a specific albumin-binding protein localized in caveolae. The activation of gp60 induces its cell surface clustering and association with caveolin-1, the caveolar-scaffolding protein. This interaction leads to G(i)-induced Src kinase activation, which in turn signals dynamin-2-mediated ... More
Regulation of caveolar endocytosis by syntaxin 6-dependent delivery of membrane components to the cell surface.
AuthorsChoudhury A, Marks DL, Proctor KM, Gould GW, Pagano RE
JournalNat Cell Biol
PubMed ID16565709
'Caveolar endocytosis has an important function in the cellular uptake of some bacterial toxins, viruses and circulating proteins. However, the molecular machinery involved in regulating caveolar uptake is poorly defined. Here, we demonstrate that caveolar endocytosis is regulated by syntaxin 6, a target membrane soluble N-ethylmaleimide attachment protein receptor (t-SNARE) ... More
Concentration and separation of proteins in microfluidic channels on the basis of transverse IEF.
AuthorsMacounová K, Cabrera CR, Yager P
JournalAnal Chem
PubMed ID11321320
'The use of microfluidic channels formed by two electrodes made of gold or palladium to perform transverse isoelectric focusing (IEF) is presented as a means for continuous concentration and fractionation of proteins. The microchannels were 40 mm long with an electrode gap of 1.27 mm and a depth of 0.354 ... More
Carbon nanotubes as intracellular protein transporters: generality and biological functionality.
AuthorsKam NW, Dai H
JournalJ Am Chem Soc
PubMed ID15839702
'Various proteins adsorb spontaneously on the sidewalls of acid-oxidized single-walled carbon nanotubes. This simple nonspecific binding scheme can be used to afford noncovalent protein-nanotube conjugates. The proteins are found to be readily transported inside various mammalian cells with nanotubes acting as the transporter via the endocytosis pathway. Once released from ... More
A method for evaluating nanoparticle transport through the blood-brain barrier in vitro.
AuthorsGuarnieri D, Muscetti O, Netti PA
Journal
PubMed ID24567140
'Blood-brain barrier (BBB) represents a formidable barrier for many therapeutic drugs to enter the brain tissue. The development of new strategies for enhancing drug delivery to the brain is of great importance in diagnostics and therapeutics of central nervous system (CNS) diseases. In this context, nanoparticles are an emerging class ... More
Non-covalent delivery of proteins into mammalian cells.
AuthorsLoudet A, Han J, Barhoumi R, Pellois JP, Burghardt RC, Burgess K,
JournalOrg Biomol Chem
PubMed ID19039359
Substances that mediate the import of proteins into cells,
Antibody to Langerin/CD207 localizes large numbers of CD8alpha+ dendritic cells to the marginal zone of mouse spleen.
AuthorsIdoyaga J, Suda N, Suda K, Park CG, Steinman RM,
JournalProc Natl Acad Sci U S A
PubMed ID19168629
Dendritic cells (DCs) are strategically positioned to take up antigens and initiate adaptive immunity. One DC subset expresses CD8alphaalpha in mice and is specialized to capture dying cells and process antigens for MHC class I ... More
Carbon nanotubes as intracellular transporters for proteins and DNA: an investigation of the uptake mechanism and pathway.
AuthorsKam NW, Liu Z, Dai H,
JournalAngew Chem Int Ed Engl
PubMed ID16345107
Herein, we present the first systematic investigation of the cellular uptake mechanism and pathway for carbon nanotubes (SWNT). We first show that intracellular transportation of proteins and DNA by SWNTs is indeed general, thus further confirming the transporter ability of these materials. We then present evidence that shows clathrin-dependent endocytosis ... More
The global spread of malaria in a future, warmer world.
AuthorsRogers DJ, Randolph SE
JournalScience
PubMed ID10976072
The frequent warnings that global climate change will allow falciparum malaria to spread into northern latitudes, including Europe and large parts of the United States, are based on biological transmission models driven principally by temperature. These models were assessed for their value in predicting present, and therefore future, malaria distribution. ... More
Tpr is localized within the nuclear basket of the pore complex and has a role in nuclear protein export.
AuthorsFrosst P, Guan T, Subauste C, Hahn K, Gerace L
JournalJ Cell Biol
PubMed ID11839768
Tpr is a coiled-coil protein found near the nucleoplasmic side of the pore complex. Since neither the precise localization of Tpr nor its functions are well defined, we generated antibodies to three regions of Tpr to clarify these issues. Using light and EM immunolocalization, we determined that mammalian Tpr is ... More
Determination of the concentration dependence of polyelectrolyte diffusion coefficients by application of the Boltzmann gradient method.
AuthorsWagner AT, Kohler HH
JournalJ Colloid Interface Sci
PubMed ID18155229
The concentration dependence of a polyelectrolyte diffusion coefficient in aqueous low salt solution (KCl, 1 mM) is determined from a single dynamic gradient experiment. The Boltzmann method is applied to calculate the diffusion coefficient. A special diffusion cell is constructed that minimizes aberrations in the optical detection of the polyion ... More
Endothelial cell-surface gp60 activates vesicle formation and trafficking via G(i)-coupled Src kinase signaling pathway.
AuthorsMinshall RD, Tiruppathi C, Vogel SM, Niles WD, Gilchrist A, Hamm HE, Malik AB
JournalJ Cell Biol
PubMed ID10973995
We tested the hypothesis that the albumin-docking protein gp60, which is localized in caveolae, couples to the heterotrimeric GTP binding protein G(i), and thereby activates plasmalemmal vesicle formation and the directed migration of vesicles in endothelial cells (ECs). We used the water-soluble styryl pyridinium dye N-(3-triethylaminopropyl)-4-(p-dibutylaminostyryl) pyridinium dibromide (FM 1-43) ... More
Ionising radiation increases permeability of endothelium through ADAM10-mediated cleavage of VE-cadherin.
AuthorsKabacik S, Raj K
JournalOncotarget
PubMed ID29137243
'The association between ionising radiation (IR) exposure and risk of cardiovascular diseases (CVD) is well documented, but the underlying mechanism is still poorly understood. As atherosclerotic plaques are the most common cause of CVD, we investigated the effects of IR on one of the critical parameters for atherosclerotic plaque formation ... More
Multifunctional biophotonic nanostructures inspired by the longtail glasswing butterfly for medical devices.
Authors
JournalNat Nanotechnol
PubMed ID29713074
Mechano-activated biomolecule release in regenerating load-bearing tissue microenvironments.
Authors
JournalBiomaterials
PubMed ID33099065
Mechanisms Driving Neutrophil-Induced T-cell Immunoparalysis in Ovarian Cancer.
Authors
JournalCancer Immunol Res
PubMed ID33990375
Mechanically-Activated Microcapsules for 'On-Demand' Drug Delivery in Dynamically Loaded Musculoskeletal Tissues.
Authors
JournalAdv Funct Mater
PubMed ID32655335
High-performance probes for light and electron microscopy.
Authors
JournalNat Methods
PubMed ID25915120
Single-cell western blotting.
Authors
JournalNat Methods
PubMed ID24880876
The Wnt Inhibitor Apcdd1 Coordinates Vascular Remodeling and Barrier Maturation of Retinal Blood Vessels.
Authors
JournalNeuron
PubMed ID29154126
Vascular and Neurogenic Rejuvenation in Aging Mice by Modulation of ASM.
Authors
JournalNeuron
PubMed ID30269989
Impaired autophagy bridges lysosomal storage disease and epithelial dysfunction in the kidney.
Authors
JournalNat Commun
PubMed ID29323117
KIM-1 mediates fatty acid uptake by renal tubular cells to promote progressive diabetic kidney disease.
Authors
JournalCell Metab
PubMed ID33951465
The presence of human mesenchymal stem cells of renal origin in amniotic fluid increases with gestational time.
AuthorsRahman MS, Spitzhorn LS, Wruck W, Hagenbeck C, Balan P, Graffmann N, Bohndorf M, Ncube A, Guillot PV, Fehm T, Adjaye J
JournalStem Cell Res Ther
PubMed ID29695308
Established therapies for managing kidney dysfunction such as kidney dialysis and transplantation are limited due to the shortage of compatible donated organs and high costs. Stem cell-based therapies are currently under investigation as an alternative treatment option. As amniotic fluid is composed of fetal urine harboring mesenchymal stem cells (AF-MSCs), ... More