Efficient and rapid derivation of primitive neural stem cells and generation of brain subtype neurons from human pluripotent stem cells.
AuthorsYan Y, Shin S, Jha BS, Liu Q, Sheng J, Li F, Zhan M, Davis J, Bharti K, Zeng X, Rao M, Malik N, Vemuri MC,
Journal
PubMed ID24113065
'Human pluripotent stem cells (hPSCs), including human embryonic stem cells and human induced pluripotent stem cells, are unique cell sources for disease modeling, drug discovery screens, and cell therapy applications. The first step in producing neural lineages from hPSCs is the generation of neural stem cells (NSCs). Current methods of ... More
New type of Sendai virus vector provides transgene-free iPS cells derived from chimpanzee blood.
AuthorsFujie Y, Fusaki N, Katayama T, Hamasaki M, Soejima Y, Soga M, Ban H, Hasegawa M, Yamashita S, Kimura S, Suzuki S, Matsuzawa T, Akari H, Era T,
Journal
PubMed ID25479600
'Induced pluripotent stem cells (iPSCs) are potentially valuable cell sources for disease models and future therapeutic applications; however, inefficient generation and the presence of integrated transgenes remain as problems limiting their current use. Here, we developed a new Sendai virus vector, TS12KOS, which has improved efficiency, does not integrate into ... More
Differentiation of Human Neural Stem Cells into Motor Neurons Stimulates Mitochondrial Biogenesis and Decreases Glycolytic Flux.
AuthorsO'Brien LC, Keeney PM, Bennett JP,
Journal
PubMed ID25892363
Differentiation of human pluripotent stem cells (hPSCs) in vitro offers a way to study cell types that are not accessible in living patients. Previous research suggests that hPSCs generate ATP through anaerobic glycolysis, in contrast to mitochondrial oxidative phosphorylation (OXPHOS) in somatic cells; however, specialized cell types have not been ... More
Induced pluripotent stem cell - derived neurons for the study of spinocerebellar ataxia type 3.
AuthorsHansen SK, Stummann TC, Borland H, Hasholt LF, Tümer Z, Nielsen JE, Rasmussen MA, Nielsen TT, Daechsel JC, Fog K, Hyttel P
JournalStem Cell Res
PubMed ID27596958
'The neurodegenerative disease spinocerebellar ataxia type 3 (SCA3) is caused by a CAG-repeat expansion in the ATXN3 gene. In this study, induced pluripotent stem cell (iPSC) lines were established from two SCA3 patients. Dermal fibroblasts were reprogrammed using an integration-free method and the resulting SCA3 iPSCs were differentiated into neurons. ... More
Induced pluripotent stem cell (iPSC) line (HEBHMUi002-A) from a healthy female individual and neural differentiation.
AuthorsMa J, Zhang J, He J, Zhang Z, Li W, Feng B, Guo R, Amponsah AE, Kong D, Liu A, Song Y, Wei L, Cui H
JournalStem Cell Res
PubMed ID31791011
'Induced pluripotent stem cells (iPSCs) can be used to generate different types of somatic cells in vitro, including neuronal cells. Here, a human iPSC line was generated from the peripheral blood mononuclear cells of a healthy 39-year-old individual. The resulting iPSCs were integration-free, maintained the normal karyotype, expressed pluripotency stem ... More
Detection of small numbers of iPSCs in different heterogeneous cell mixtures with highly sensitive droplet digital PCR.
'Induced pluripotent stem cells (iPS cells) are a prospective resource for regenerative biomedicine. iPS cells can differentiate into any type of stem, progenitor and somatic cells to help replace structures within damaged organs or tissues. However, iPS cells themselves, can produce malignant tumors if they are injected into the body ... More
Bioink Composition and Printing Parameters for 3D Modeling Neural Tissue.
AuthorsFantini V, Bordoni M, Scocozza F, Conti M, Scarian E, Carelli S, Di Giulio AM, Marconi S, Pansarasa O, Auricchio F, Cereda C
JournalCells
PubMed ID31387210
'Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system. The main problem in the study of NDs is the lack of an adequate realistic experimental model to study the pathogenic mechanisms. Induced pluripotent stem cells (iPSCs) partially overcome ... More
Neuronal differentiation defects in induced pluripotent stem cells derived from a Prader-Willi syndrome patient.
AuthorsSoeda S, Saito R, Fujita N, Fukuta K, Taniura H
JournalNeurosci Lett
PubMed ID30902571
'Prader-Willi syndrome (PWS) is a neurodevelopmental disorder caused by a lack of expression of paternally inherited genes located in the15q11.2-q13 chromosome region. An obstacle in the study of human neurological diseases is the inaccessibility of brain material. Generation of induced pluripotent stem cells (iPSC cells) from patients can partially overcome ... More
TDP-43 and NOVA-1 RNA-binding proteins as competitive splicing regulators of the schizophrenia-associated TNIK gene.
AuthorsGumina V, Colombrita C, Fallini C, Bossolasco P, Maraschi AM, Landers JE, Silani V, Ratti A
JournalBiochim Biophys Acta Gene Regul Mech
PubMed ID31382054
'The RNA-binding protein TDP-43, associated to amyotrophic lateral sclerosis and frontotemporal dementia, regulates the alternative splicing of several genes, including the skipping of TNIK exon 15. TNIK, a genetic risk factor for schizophrenia and causative for intellectual disability, encodes for a Ser/Thr kinase regulating negatively F-actin dynamics. Here we show ... More
c-Jun N-terminal kinase (JNK)-mediated phosphorylation of SARM1 regulates NAD
AuthorsMurata H, Khine CC, Nishikawa A, Yamamoto KI, Kinoshita R, Sakaguchi M
JournalJ Biol Chem
PubMed ID30333228
Mitochondrial dysfunction is a key pathological feature of many different types of neurodegenerative disease. Sterile alpha and Toll/interleukin receptor motif-containing protein 1 (SARM1) has been attracting much attention as an important molecule for inducing axonal degeneration and neuronal cell death by causing loss of NAD (NADH). However, it has remained ... More
Autophagy Induction by Bexarotene Promotes Mitophagy in Presenilin 1 Familial Alzheimer's Disease iPSC-Derived Neural Stem Cells.
AuthorsMartín-Maestro P, Sproul A, Martinez H, Paquet D, Gerges M, Noggle S, Starkov AA
JournalMol Neurobiol
PubMed ID31203573
Adult neurogenesis defects have been demonstrated in the brains of Alzheimer's disease (AD) patients. The neurogenesis impairment is an early critical event in the course of familiar AD (FAD) associated with neuronal loss. It was suggested that neurologic dysfunction in AD may be caused by impaired functioning of hippocampal neural ... More
Generation of human iPS cell line CBTCi001-A from dermal fibroblasts obtained from a healthy donor.
AuthorsMartins GLS, Paredes BD, Sampaio GLA, Nonaka CKV, da Silva KN, Allahdadi KJ, França LSA, Soares MBP, Dos Santos RR, Souza BSF
JournalStem Cell Res
PubMed ID31706097
Human-induced pluripotent stem cell (hiPSC) CBTCi001-A line was generated from a healthy 30-year old male dermal fibroblasts using non-integrative reprogramming method using episomal-based plasmids expressing OCT4, SOX2, KLF4, and MYCL. Characterization of CBTCi001-A was confirmed by the expression of typical markers of pluripotency and differentiation potential in vitro. ... More
Typical halogenated flame retardants affect human neural stem cell gene expression during proliferation and differentiation via glycogen synthase kinase 3 beta and T3 signaling.
AuthorsLiang S, Liang S, Zhou H, Yin N, Faiola F
JournalEcotoxicol Environ Saf
PubMed ID31377521
2',2',4,4'-tetrabromo diphenyl ether (BDE-47), one of the most abundant congeners of commercial pentaBDE utilized as flame retardants, has been phased out of production due to its potential neural toxicity and endocrine disrupting activities, and yet still present in the environment. Several alternatives to BDE-47, including tetrabromobisphenol A (TBBPA), tetrabromobisphenol S ... More
Modelling Neurotropic Flavivirus Infection in Human Induced Pluripotent Stem Cell-Derived Systems.
AuthorsDesole G, Sinigaglia A, Riccetti S, Masi G, Pacenti M, Trevisan M, Barzon L
JournalInt J Mol Sci
PubMed ID31671583
Generation of human induced pluripotent stem cells (hiPSCs) and their differentiation into a variety of cells and organoids have allowed setting up versatile, non-invasive, ethically sustainable, and patient-specific models for the investigation of the mechanisms of human diseases, including viral infections and host-pathogen interactions. In this study, we investigated and ... More
Reprogramming and differentiation-dependent transcriptional alteration of DNA damage response and apoptosis genes in human induced pluripotent stem cells.
AuthorsShimada M, Tsukada K, Kagawa N, Matsumoto Y
JournalJ Radiat Res
PubMed ID31665364
Pluripotent stem cells (PSCs), such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), have a dual capability to self-renew and differentiate into all cell types necessary to develop an entire organism. Differentiation is associated with dynamic epigenetic alteration and transcriptional change, while self-renewal depends on maintaining the ... More
Vascular progenitors generated from tankyrase inhibitor-regulated naïve diabetic human iPSC potentiate efficient revascularization of ischemic retina.
AuthorsPark TS, Zimmerlin L, Evans-Moses R, Thomas J, Huo JS, Kanherkar R, He A, Ruzgar N, Grebe R, Bhutto I, Barbato M, Koldobskiy MA, Lutty G, Zambidis ET
JournalNat Commun
PubMed ID32139672
Here, we report that the functionality of vascular progenitors (VP) generated from normal and disease-primed conventional human induced pluripotent stem cells (hiPSC) can be significantly improved by reversion to a tankyrase inhibitor-regulated human naïve epiblast-like pluripotent state. Naïve diabetic vascular progenitors (N-DVP) differentiated from patient-specific naïve diabetic hiPSC (N-DhiPSC) possessed higher ... More
Bisphenol A and several derivatives exert neural toxicity in human neuron-like cells by decreasing neurite length.
AuthorsLiang X, Yin N, Liang S, Yang R, Liu S, Lu Y, Jiang L, Zhou Q, Jiang G, Faiola F
JournalFood Chem Toxicol
PubMed ID31812737
Bisphenol A (BPA) and its derivatives, including bisphenols S (BPS), F (BPF), E (BPE), B (BPB), Z (BPZ), and AF (BPAF), are widely used in consumer products. Moreover, they are typically detected in the environment, food, and humans. Previous studies have linked BPA to several health risks, but it is ... More