After generating neural stem cells (NSCs) using PSC Neural Induction Medium, confirm expression of NSC markers with this convenient multicolor immunocytochemistry kit.

PSC Neural Induction Medium

Catalog number: A1647801

Gibco™  Related applications: Clinical & Translational Research | Mammalian Cell Culture | Primary Cell Culture | Stem Cell Differentiation

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Description

Gibco® PSC Neural Induction Medium is a serum-free medium that provides high efficiency neural induction of human pluripotent stems cells (PSCs) in only 7 days. Unlike existing methodologies, use of PSC Neural Induction Medium does not require the intermediary step of embryoid body (EB) formation, which adds time, labor, and variability. Neural Stem Cells (NSCs) generated using PSC Neural Induction Medium have high expression of NSC markers and can be further differentiated into other neural cell types.

PSC Neural Induction Medium enables:

Rapid induction of NSCs—high efficiency neural induction in only 7 days, without EB formation
Scalable production of NSCs:—generate >20 million NSCs starting from 1 million PSCs
Generation of high quality NSCs—resulting NSCs can be cryopreserved, expanded, and differentiated

Rapid Induction of NSCs
PSC Neural Induction Medium simplifies the NSC generation workflow by eliminating the need to form EBs or rosettes. By removing this step, PSC Neural Induction Medium enables a more reproducible process with high efficiency neural induction in only 7 days.

Scalable Production of NSCs
PSC Neural Induction Medium is a robust medium that has been shown to generate over 20 million NSCs from 1 million PSCs. The medium has been tested with multiple hPSC lines including hiPSCs derived from use of Episomal iPSC Reprogramming Vectors and the CytoTune®-iPS Sendai Reprogramming Kit.

Generation of High Quality NSCs
Neural stem cells generated using PSC Neural Induction Medium have >80% expression of Sox1, Sox2, and Nestin (NSC markers), and <1% expression of Oct4 (pluripotency marker). In addition, expanded NSCs maintained phenotype, exhibited normal karyotype, and could be differentiated into neurons, astrocytes, and oligodendrocytes.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Stem Cells (Neural), Stem Cells (Embryonic), Stem Cells (Human), Stem Cells (iPS - Induced Pluripotent Stem), Stem Cells
Product Size: 500 mL

Contents & storage

Contains:
• 500 mL Basal medium (store at 2–8°C)
• 10 mL Supplement (store at -5 to -30°C)

Documents

Manuals & protocols

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