Alignflow™ Flow Cytometry Alignment Beads for Red Lasers, 2.5 µm

Catalog number:  A16501

Related applications:

Flow Cytometry

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Molecular Probes® AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 2.5 µm Alignflow™ Flow Cytometry Alignment Beads for Red Lasers are fluorescent-dye infused microspheres that are excitable at 630–640 nm with emission in the range of 645–680 nm.

View information for all flow cytometry calibration and size reference beads.

AlignFlow™ beads provide:

• Uniformity—very low FSC CV and intensity CV
• Consistency in production, creating ideal reference standards
• Confidence through reliable, optimal instrument performance
• Compatibility—use with any instrument

The AlignFlow™ beads are highly uniform with respect to size and fluorescence intensity, resulting in low FSC CV and intensity CV. The beads approximate the size, emission wavelength, and intensity of many biological samples and permit the calibration of the flow cytometer’s laser source, optics, and stream flow without wasting valuable and sensitive experimental material.

Bead Labeling
As with all of our flow cytometry standard microspheres, the AlignFlow™ beads are stained internally rather than on the surface. The dyes are therefore insulated from environmental interactions that could cause variable fluorescence output, resulting in excellent signal stability. These fluorescent polystyrene microspheres are supplied as suspensions packaged in dropper vials for convenient dispensation and are available in a choice of three colors (UV, blue, red) and two sizes (2.5 µm, 6.0 µm) to match your laser.

The AlignFlow™ flow cytometry reference beads are provided in updated packaging that includes a Quick Reference Label that puts key information for product use at your fingertips.

These beads replace previous AlignFlow™ flow cytometry reference bead products. This particular product replaces AlignFlow™ Flow Cytometry Alignment Beads, 2.5 µm, for 633 excitation (A7312).
For Research Use Only. Not for use in diagnostic procedures.


Calibration Type: Flow Cytometry Alignment Beads
Detection Method: Fluorescent
Flow Cytometer Laser Lines: 633 or 635
Form: Suspension
Product Line: AlignFlow™
Shipping Condition: Room Temperature
Stain Configuration: Core
Diameter (Metric): 2.5 µm
For Use With (Equipment): Flow Cytometer

Contents & storage

Contains 2 vials of Alignflow™ beads (1.5 mL per vial). Store at 2–8°C.