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Invitrogen
Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647
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FIGURE: 1 / 2
Goat IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21446) in WB
Multiplexed fluorescent western blot was performed using Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 (Product # A-21446). Membrane enriched extracts of LNCap (Lane 1, 2, 3, 4), PC-3 (Lane 5) and HeLa (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were transferred onto anitrocellulose membrane (Product # IB230... View More
Product Details
A-21446
Applications
Tested Dilution
Western Blot (WB)
1:5000-1:10,000
Immunohistochemistry (IHC)
1-10 µg/mL
Immunocytochemistry (ICC/IF)
1-10 µg/mL
Product Specifications
Species Reactivity
Goat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobins Heavy and Light chains
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
2 mg/mL
Amount
1 mg
Purification
purified
Storage buffer
PBS, pH 7.5
Contains
5mM sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG
Cross Adsorption
Against human and rat serum proteins prior to conjugation
Antibody Form
Whole Antibody
Product Specific Information
Fluorescence of this long-wavelength Alexa Fluor dye is not visible by looking through a conventional fluorescence microscope.
Product will be shipped at Room Temperature.
Target Information
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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