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Bethyl Laboratories
Aurora B Polyclonal Antibody
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FIGURE: 1 / 3
Aurora B Antibody (A300-431A) in ICC/IF
Detection of human Aurora B by immunocytochemistry. Samples: Asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Aurora B antibody BL1075 (Product # A300-431A) used at 0.5 µg/mL in A and at 2 µg/mL in B. Detection: Secondary antibodies were anti-rabbit IgG conjugated to FITC in A and conjugated to Texas Red in B.
Product Details
A300-431A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 300 and the C-terminus (residue 344) of human Aurora B kinase.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
The Aurora kinases associate with microtubules during chromosome movement and segregation. AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AIM-1; ARK-2; Aurora 1; Aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora- and IPL1-like midbody-associated protein 1; aurora- and Ipl1-like midbody-associated protein 1 homolog; aurora-1; aurora-B; aurora-related kinase 2; Aurora/IPL1-related kinase 2; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase aurora-B; STK-1; unnamed protein product
Gene Aliases: AIK2; AIM-1; AIM1; AIRK2; ARK-2; ARK2; AurB; AURKB; aurkb-sv1; aurkb-sv2; IPL1; PPP1R48; STK-1; STK1; STK12; STK5
UniProt ID: (Human) Q96GD4
Entrez Gene ID: (Human) 9212
negative regulation of transcription from RNA polymerase II promoter
mitotic cell cycle
mitotic cytokinesis
negative regulation of B cell apoptotic process
spindle organization
mitotic spindle organization
positive regulation of microtubule depolymerization
positive regulation of telomere maintenance
regulation of cytokinesis
negative regulation of cytokinesis
positive regulation of cytokinesis
regulation of microtubule-based process
protein localization to kinetochore
cellular response to UV
cleavage furrow formation
post-translational protein modification
cell cycle G2/M phase transition
mitotic cytokinesis checkpoint
negative regulation of innate immune response
mitotic spindle midzone assembly
cell division
attachment of mitotic spindle microtubules to kinetochore
regulation of chromosome segregation
midbody abscission
positive regulation of mitotic sister chromatid segregation
positive regulation of cell cycle process
positive regulation of mitotic cell cycle spindle assembly checkpoint
mitotic spindle assembly
repair of mitotic kinetochore microtubule attachment defect
cGAS/STING signaling pathway
negative regulation of cGAS/STING signaling pathway
regulation of signal transduction by p53 class mediator
positive regulation of mitotic sister chromatid separation
positive regulation of attachment of mitotic spindle microtubules to kinetochore
positive regulation of mitotic cytokinesis
positive regulation of lateral attachment of mitotic spindle microtubules to kinetochore
mitotic sister chromatid biorientation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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