Pierce™ Proteaseinhibitor in Tablettenform, EDTA-frei
Pierce™ Proteaseinhibitor in Tablettenform, EDTA-frei
Thermo Scientific™

Pierce™ Proteaseinhibitor in Tablettenform, EDTA-frei

Thermo Scientific Pierce Protease Inhibitor -Tabletten, EDTA-frei, enthalten breitgefächerte Protease-Inhibitoren, die den proteolytischem Abbau bei Zelllyse- und Proteinextraktionsuntersuchungen sehr wirksamWeitere Informationen
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KatalognummerMenge
A3296520 Tabletten
Katalognummer A32965
Preis (EUR)
355,65
Exklusiv online
409,00
Ersparnis 53,35 (13%)
Each
Zum Warenkorb hinzufügen
Menge:
20 Tabletten
Preis (EUR)
355,65
Exklusiv online
409,00
Ersparnis 53,35 (13%)
Each
Zum Warenkorb hinzufügen
Thermo Scientific Pierce Protease Inhibitor -Tabletten, EDTA-frei, enthalten breitgefächerte Protease-Inhibitoren, die den proteolytischem Abbau bei Zelllyse- und Proteinextraktionsuntersuchungen sehr wirksam verhindern.

Merkmale von EDTA-freien Protease-Inhibitor-Tabletten:
Verbesserte Formulierung – Tabletten lösen sich schnell zu einer klaren Lösung auf
Wirtschaftlich – Kostengünstiger als andere handelsübliche Rezepturen für gleichwertige Mengen
Praktisch – gebrauchsfertige, vollständig offengelegte Breitspektrum-Formulierungen
Kompatibel – direkt mit Pierce Protein-Assays einsetzbar
EDTA-frei – formuliert ohne EDTA, einem Metalloproteinase-Inhibitor (nur für EDTA-freie Formulierungen)

Pierce Protease Inhibitor -Tabletten schützen Proteine vor proteolytischem Abbau durch Hemmung von Serin-Proteasen, Cystein-Proteasen, Asparaginsäure-Proteasen und Aminopeptidase, die in der Regel in Zelllysat-Proben vorhanden sind.Die Tabletten enthalten AEBSF, Aprotinin, Bestatatin, E-64, Leupeptin und Pepstatin A. Sie sind bei 4 °C bis zu 12 Monate lang stabil. Jede Tablette reicht für 50 ml Lösung und eignet sich für die Reagenzienvorbereitung vor der Proteinextraktion aus Gewebe und kultivierten Zellen.

Zur großen Enzymfamilie der Proteasen gehören auch die Endo- und Exopeptidasen, die für den hydrolytischen Abbau von Proteinen in Peptide oder Aminosäuren verantwortlich sind.Proteasen befinden sich in Zellkompartimenten, aber nach der Zelllyse werden die Proteasen freigesetzt, was zu einem Abbau wertvoller Proteine führt.Aus diesem Grund ist es gängige Praxis, Zelllyse-Puffer bzw. extrahierte Zellen mit chemische Verbindungen, die Proteasen deaktivieren bzw. hemmen, zu versetzen.

Um Pierce Protease Inhibitor-Tabletten zu verwenden, lösen Sie einfach 1 Tablette in 50 ml Puffer oder Lysat auf.Diese Rezeptur zur Proteasehemmung ist mit den meisten Detergenzien-basierten Zelllysereagenzien einschließlich Pierce Cell Lysis-Lösungen kompatibel.Pierce Protease Inhibitor Tablets enthalten keine Phosphatase-Inhibitoren.Falls erforderlich, können Phosphatase-Inhibitoren wie Pierce Phosphatase Inhibitor-Minitablettenzusammen mit Protease-Inhibitor-Tabletten hinzugefügt werden.

Zugehörige Produkte
Pierce Protease Inhibitor-Tabletten
Pierce Protease Inhibitor-Minitabletten EDTA-frei
Pierce Phosphatase Inhibitor-Minitabletten
Pierce Protease und Phosphatase Inhibitor Minitabletten

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications
HemmstoffeProtease-Inhibitor
Menge20 Tabletten
VersandbedingungRaumtemperatur
Ausreichend für50 ml Lösung
FormFest
ProduktliniePierce
ProdukttypProtease-Inhibitor-Cocktail
Unit SizeEach
Inhalt und Lagerung
1 Fläschchen, bei 2 bis 8 °C lagern

Häufig gestellte Fragen (FAQ)

Can I use just part of the Pierce Protease Inhibitor XL Capsules, EDTA-Free and save part of the capsule for later use?

We do not recommend “splitting” the capsule into smaller portions. Researchers using less than 500 mL should choose a different format (liquid or tablet) of protease inhibitors.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What formats of protease inhibitors do you offer?

We offer standalone protease inhibitors (AEBSF, Aprotinin, PMSF, EDTA, etc.), as well as cocktails or combinations of protease inhibitors in liquid, tablet, and larger capsule formats. These cocktails of protease inhibitors can also be purchased in combination with a cocktail of phosphatase inhibitors, in both liquid and tablet form, for even greater convenience.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer any of the components of the Pierce protease, phosphatase and (protease and phosphatase) inhibitor tablets and mini tablets individually?

Yes, the following components of the Pierce protease, phosphatase and (protease and phosphatase) inhibitor tablets and mini tablets are offered separately:

- AEBSF (Cat. No. 78431)
- Aprotinin (Cat. No. 78432)
- Bestatin (Cat. No. 8433)
- E-64 (Cat. No. 78434)
- Leupeptin (Cat. No. 78435)
- Pepstatin A (Cat. No. 78436)
- EDTA (Cat. No. 17892).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the protease and phosphatase inhibitor tablets and mini tablets?

The final concentration of each component in the protease and phosphatase inhibitor tablets and mini tablets is proprietary. We release only the identities of the inhibitors.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I store the reconstituted solution of protease, phosphatase and (protease and phosphatase) inhibitor tablet or mini tablet and if so, for how long?

The Pierce protease, phosphatase, as well as (protease and phosphatase) inhibitor tablets and mini tablets are stable for at least one year when stored at 4 degrees C, but we only recommend preparing fresh solutions just before needed. If you are interested in liquid protease or phosphatase inhibitors, please use our inhibitor cocktails (https://www.thermofisher.com/search/results?query=protease%20inhibitor%20cocktails&focusarea=Search%20All).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Zitierungen und Referenzen (3)

Zitierungen und Referenzen
Abstract
An Introductory Guide to Protease Sensitive Linker Design Using Matrix Metalloproteinase 13 as an Example.
Authors:Hamm P,Meinel L,Driessen MD
Journal:ACS biomaterials science & engineering
PubMed ID:38813796
Proteases play a crucial role, not only in physiological, but also in pathological processes, such as cancer, inflammation, arthritis, Alzheimer's, and infections, to name but a few. Their ability to cleave peptides can be harnessed for a broad range of biotechnological purposes. To do this efficiently, it is essential to ... More
Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions.
Authors:Wang K,Escobar M,Li J,Mahata B,Goell J,Shah S,Cluck M,Hilton IB
Journal:Nucleic acids research
PubMed ID:35849129
Nuclease-inactivated CRISPR/Cas-based (dCas-based) systems have emerged as powerful technologies to synthetically reshape the human epigenome and gene expression. Despite the increasing adoption of these platforms, their relative potencies and mechanistic differences are incompletely characterized, particularly at human enhancer-promoter pairs. Here, we systematically compared the most widely adopted dCas9-based transcriptional activators, ... More
Not just fat: investigating the proteome of cetacean blubber tissue.
Authors:Kershaw JL,Botting CH,Brownlow A,Hall AJ
Journal:Conservation physiology
PubMed ID:29479430
Mammalian adipose tissue is increasingly being recognized as an endocrine organ involved in the regulation of a number of metabolic processes and pathways. It responds to signals from different hormone systems and the central nervous system, and expresses a variety of protein factors with important paracrine and endocrine functions. This ... More