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Poly-D-Lysine - FAQs

Poly-D-Lysine - FAQs

View additional product information for Poly-D-Lysine - FAQs (A3890401)

10 product FAQs found

What solution is Poly-D-Lysine (Cat. No. A3890401) delivered in?

Poly-D-Lysine is in PBS solution.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I freeze Poly-D-Lysine?

Yes, Poly-D-Lysine can undergo one freeze-thaw cycle without impacting performance. Please note that this does not extend the shelf-life of the product.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are there any animal origin components in Poly-D-Lysine?

The Poly-D-Lysine matrix is animal origin-free.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I coated my plates with Poly-D-Lysine and I'm noticing that the cells are dying within a few days of plating the cells. How can I ensure cell survival?

It is possible that the plates were not adequately rinsed with water prior to plating the cells. Residual Poly-D-Lysine is toxic and may be the cause of cell death. It is recommended to rinse the plates with water three times before exposing the cells to the matrix.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I coated my plates with Poly-D-Lysine and I'm noticing that the cells are not attaching well. What can I do to prevent the cells from lifting off?

When coating the surface with Poly-D-Lysine, make sure that the entire surface is fully covered and that the appropriate volume of coating matrix is applied.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What type of cultureware can I pair with Poly-D-Lysine?

The Poly-D-Lysine matrix has been coated on a variety of Nunc cell cultured treated plastic multi-well dishes as well as glass surfaces.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use the Poly-D-Lysine matrix with other coatings?

iPSC-derived neurons have been cultured successfully on Poly-D-Lysine with a secondary coating of mouse and human laminin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?

We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What cell types will Poly-D-Lysine support as an attachment matrix?

The Poly-D-Lysine matrix has successfully supported the attachment of primary rodent neurons, iPSC-derived neurons and PC12 cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Poly-D-Lysine at 4 degrees C?

The product can be stored at 4 degrees C for 12 months after the date of production.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.